National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chitinases – the primary chitinolytic enzymes have gained great attention due to its industrial, environmental, agricultural, medicinal and biotechnological applications. Nevertheless, the industrial-scale production of this enzyme remains in its early stages due to low yielding strains and high cost of production. This study focuses on enhancing chitinase production from low- cost chitin substrate through cumulative optimization, harnessing a high-yielding novel chitinase-producing strain isolated from Mimosa pudica root nodules. Among the various isolates, the best chitinase producer, identified as Paenibacillus yonginensis, exhibited an inherent high chitinase activity of 1.08 U/100 ml. Using Minitab statistical software, contemporary statistical optimization techniques were applied to optimize the media for Paenibacillus yonginensis chitinase production. Through Plackett-Burman design, it was determined that various factors including substrate (colloidal chitin), incubation time and pH were notably significant. Further optimization with a central composite design (CCD) approach using Response Surface Methodology (RSM) resulted in improved medium composition that yielded chitinase production up to 2.22 U/100 ml. It would be advantageous to use a high chitinase producing isolate in conjunction with the inexpensive and reliable nature of chitin as a carbon source to produce constant enzyme yields.
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