National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study reports an eco-friendly green synthesis of chitosan nanoparticles (CSNPs) using lemon (Citrus limon) juice as a natural reducing and stabilizing agent, and evaluates their dual functionality in enhancing microbial indole-3-acetic acid (IAA) production and inhibiting a Gram-negative pathogen. The synthesized CSNPs were characterized by UV–Vis spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray analysis, confirming stable nanoparticle formation with spherical morphology and particle sizes in the range of 100- 200 nm. Functional group analysis indicated successful citric-acid-mediated cross-linking and incorporation of lemon-derived phytochemicals. Biological evaluation demonstrated that CSNP supplementation significantly enhanced IAA production in Azotobacter sp. and Pseudomonas aeruginosa, resulting in increases of 91.6% and 99.8%, respectively, compared to untreated controls. In addition, CSNPs exhibited antibacterial activity against P. aeruginosa. To gain mechanistic insight into the antimicrobial effect, in silico molecular docking was performed, revealing strong binding affinity of a chitosan oligomer toward outer membrane porins, particularly OprD, suggesting potential disruption of porin-mediated transport. Overall, the findings demonstrate that green-synthesized CSNPs possess multifunctional properties, simultaneously promoting beneficial microbial auxin production and suppressing pathogenic bacteria. These characteristics highlight their potential application as sustainable biofertilizer enhancers and eco-friendly antimicrobial agents in agricultural systems.
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