Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 14, Issue:7, July, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(7): 221-228
DOI: https://doi.org/10.20546/ijcmas.2025.1407.027


HPLC Profiling of Viscosin Producing Fluorescent pseudomonads Isolated From Sugarbeet -Maize Ecosystem Enhances Plant Growth Promotion
Radhajeyalakshmi Raju1*, Sethuraman Kandhasamy2, Sathyasheela Veluchamy3, Selvakumar Thambiyannan3 and Satheesh Kumar Natesan3
1Coconut Research Station, Aliyarnagar – 642 101, India 2Department of Plant Pathology, Anbil Dharmalingam Agricultural College and Research Institute, Tamil Nadu Agricultural University, Tiruchirappalli -620 027, India 3Maize Research Station, Tamil Nadu Agricultural University, Vagarai-624 613, India
*Corresponding author
Abstract:

Antimicrobial biosurfactant producing Fluorescent Pseudomonads biovar (Pf-V1) was isolated from Maize/Sugar beet rhizosphere in sandy loam soil and tested for the presence of viscosinby HPLC analysis. The strain performed its antifungal activity against major fungal disease of maize with zoosporicidal activity against downy mildew pathogen of maize. The strain is grouped under V1 (Associated with the production of Viscosin). The strain improves growth & systemic protection of maize crop against foliar & soil-borne pathogens and surprisingly drought tolerance. Genetic improvement of Pf strains with increased Viscosinproduction, biosurfactant properties & wide applicability to other crops will be aimed as separate research theme.


Keywords: Biosurfactant, Fluorescent pseudomonads


References:

Artursson, V., Finlay, R.D and Jansson, J. K. 2006. Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environmental Microbiology.8: 1-10. https://doi.org/10.1111/j.1462-2920.2005.00942  

Biniarz, P., ?ukaszewicz, M., Janek, T. 2017. Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review. Crit Rev Biotechnol.37(3):393-410. https://doi.org/10.3109/07388551.2016.1163324.

Bruine de Bruin, W., Parker, A.M., Fischhoff, B. 2007. Individual differences in adult decision-making 221 competence. J. Person. Soc. Psychol. 92(5):938-956. http://dx.doi.org/10.1037/0022-3514.92.5.938

Cesa-Luna, C., Geudens, N., Girard, L., De Roo, V., Maklad, H.R. 2023. Martins J, De Mot, R. Charting the lipopeptidome of nonpathogenic Pseudomonas. MSYSTEMS.8(1).https://doi.org/10.1128/msystems.00988-22 

Chauhan, V., Mazumdar, S., Pandey, A., Kanwar, S.S. 2023. Pseudomonas lipopeptide an excellent biomedical agent. Med Comm – Biomater Appl.; 2:27. https://doi.org/10.1002/mba2.CHAUHAN et al., | 15 of 15 Page 9 of 11 The Plant Pathology Journal

de Bruijn, I., De Kock, M.J.D., Yang, M., De Waard, P., Van Beek, T.A and Raaijmakers, J.M. 2007. Genome based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species. Mol Microbio.63: 417–428. https://doi.org/10.1111/j.1365-2958.2006.05525.x

Fardami, A.Y., Kawo, A.H., Yahaya, S., Lawal, I., Abubakar, A.S., Maiyadi, K.A., 2022. Review on Biosurfactant Properties, Production and Producing Microorganisms. Journal of Biochemistry, Microbiology and Biotechnology.10 (1): 5–12. https://doi.org/10.54987/jobimb.v10i1.656 

Fechtner, J., Koza, A., Sterpaio, P.D, Hapca, S.M and Spiers, A.J. 2011. Surfactants expressed by soil pseudomonads alter local soil-water distribution, suggesting a hydrological role for these compounds. FEMS Microbiol Ecol.78: 50–58. https://doi.org/10.1111/j.1574-6941.2011.01141.x

Geudens, N., Martins, J.C., 2018. Cyclic Lipodepsipeptides from Pseudomonas spp. - Biological Swiss Army Knives. Front Microbiol.Aug;14(9) :1867. https://doi.org/10.3389/fmicb.2018.01867

Groupé, V., Pugh, L., Weiss, D., Kochi M. 1951. Observations on antiviral activity of viscosin. Proc Soc Exp Biol Med.1951;78:354–358 https://doi.org/10.3181/00379727-78-19071

Haas, D and Défagon, G. 2005. Biological control of soilborne pathogens by fluorescent 236 pseudomonads. Nat Rev Microbiol.3: 307–319 https://doi.org/10.1038/nrmicro1129

Laycock, M.V., Hildebrand, P.D., Thibault, P., Walter, J.A and Wright, J.L.1991. Viscosin, a potent peptidolipid biosurfactant and phytopathogenic mediator produced by a pectolytic strain of Pseudomonas fluorescens. J Agric Food Chem.39: 483–489. Page 10 of 11 The Plant Pathology Journal Only

Mazzola, M., de Bruijn, I., Cohen, M.F and Raaijmakers, J.M. 2009. Protozoan-induced regulation of cyclic lipopeptide biosynthesis is an effective predation defense mechanism for Pseudomonas fluorescens. Appl.Environ.Microbiol.75: 6804–6811. https://doi.org/10.1128/AEM.01272-09

Neu, T.R., Härtner, T & Poralla, K.1990. Surface active properties of viscosin: a peptidolipid antibiotic. Appl. Microbiol. Biotechnol.32; 518–520. https://doi.org/10.1007/BF00173720 

O'Toole, G.A., Pratt, L.A., Watnick, P.I., Newman, D.K., Weaver, V.B. & Kolter.R. 1999. Genetic approaches to the study of biofilms. Methods Enzymol.310:91-109. https://doi.org/10.1016/s0076-6879(99)10008-9

Raaijmakers, J.M., De Bruijn I., Nybroe O and Ongena, M. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiol Rev.34: 1037–1062            https://doi.org/10.1111/j.1574-6976.2010.00221.x

Radhajeyalakshmi Raju., Sethuraman Kandhasamy., Ganesan Kalipatty Nalliappan., Kumari Vinodhana Natarajan., Karthikeyan Gandhi and Bharathi Chandrasekaran. 2016. "Cyclic depsipeptide producing fluorescent pseudomonads exerts antifungal activity against fungal pathogens of maize (Zea mays). African Journal of Microbiology Research.10(42): 1767-1774. http://dx.doi.org/10.5897/AJMR2016.8265

Salek, K. and Euston, S.R. 2019. Sustainable microbial biosurfactants and bioemulsifiers for commercial exploitation. Process Biochemistry.85:143-155. https://doi.org/10.1016/j.procbio.2019.06.027 

Shekhar, S., Sundaramanickam, A., Balasubramanian, T. 2015. Biosurfactant Producing Microbes and their Potential Applications: A Review. Critical Reviews in Environmental Science and Technology.45 (14): 1522 – 1554.   https://doi.org/10.1080/10643389.2014.955631

Singh, P., Singh, R.K., Zhou, Y., Wang, J., Jiang, Y., Shen, N. 2022. Unlocking the strength of plant growth promoting Pseudomonas in improving crop productivity in normal and challenging environments: a review. Journal of Plant Interactions.17:220-238. https://doi.org/10.1080/17429145.2022.2029963

Download this article as Download

How to cite this article:

Radhajeyalakshmi Raju, Sethuraman Kandhasamy, Sathyasheela Veluchamy, Selvakumar Thambiyannan and Satheesh Kumar Natesan. 2025. HPLC Profiling of Viscosin Producing Fluorescent pseudomonads Isolated From Sugarbeet -Maize Ecosystem Enhances Plant Growth Promotion.Int.J.Curr.Microbiol.App.Sci. 14(7): 221-228. doi: https://doi.org/10.20546/ijcmas.2025.1407.027
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations