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Original Research Articles                      Volume : 14, Issue:7, July, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
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Int.J.Curr.Microbiol.App.Sci.2025.14(7): 122-131
DOI: https://doi.org/10.20546/ijcmas.2025.1407.016


Microbial β-Galactosidases: Potential Industrial Applications
Kalyani Neti1* and Swati A. Peshwe2
1Department of Microbiology, Government Institute of Science, Ch.Sambhaji Nagar, India
2Department of Microbiology, Department of Microbiology, Government Institute of Science, Ch.Sambhaji Nagar, India
*Corresponding author
Abstract:

β-Galactosidase, (EC.3.2.1.23), most commonly known as lactase, which hydrolyses lactose into its glucose and galactose has potential applications in the dairy and food processing industries. This enzyme holds importance in formulating lactose-hydrolyzed products from both milk and whey for lactose-intolerant people. The enzyme is also crucial in biosynthesis of galacto-oligosaccharides as prebiotics for use in probiotics to restore gut health. Whey, the major dairy industry affluent can be used as a potent raw material for the growth of β -galactosidase producing microbes and the enzyme can further be used to hydrolyze whey thus reducing burden on soil and aquatic ecosystems. Free and immobilized preparations of β galactosidases have been exploited as biosensors in various applications such as industrial, biotechnological, medical, analytical areas. This review focuses on various sources of β-galactosidases, aspects of production, purification and immobilization of the enzyme and potential biotechnological applications.


Keywords: β-Galactosidase, Lactose hydrolysis, Whey, GOS, Biosensors


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Kalyani Neti and Swati A. Peshwe. 2025. Microbial β-Galactosidases: Potential Industrial Applications.Int.J.Curr.Microbiol.App.Sci. 14(7): 122-131. doi: https://doi.org/10.20546/ijcmas.2025.1407.016
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