Silver nanoparticles (AgNPs) have garnered significant interest due to their potent antibacterial properties against various pathogens. This study investigates the antibacterial activity of synthesized AgNPs against a range of Gram-positive and Gram-negative bacterial strains, including Staphylococcus aureus, Staphylococcus epidermidis, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Enterobacter aerogenes, and Salmonella typhimurium. The antibacterial efficacy was evaluated using the well diffusion method, with zones of inhibition (ZOI) recorded for each bacterial strain. The results demonstrated that Staphylococcus aureus exhibited the highest susceptibility to AgNPs (21 ± 1.8 mm), while Pseudomonas aeruginosa showed the lowest susceptibility (12 ± 1.0 mm). Gram-positive bacteria were generally more susceptible to AgNPs than Gram-negative bacteria, likely due to differences in cell wall structure and composition. These findings corroborate previous studies indicating that AgNPs disrupt bacterial cell membranes, generate reactive oxygen species (ROS), and interfere with cellular processes. The broad-spectrum antibacterial activity of AgNPs highlights their potential for applications in biomedical fields, such as wound healing, antibacterial coatings, and water purification systems. Further research is recommended to optimize AgNP synthesis and dosage for enhanced efficacy and reduced bacterial resistance.

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