National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The endo-rhizospheric microbiome plays a vital role in improving plant tolerance to abiotic stresses such as salinity. This study focuses on the morphological characterization and molecular identification of actinobacteria from the endo-rhizosphere of rice (Oryza sativa) of different doses of N applied soils. A total of 15 Actinobacterial isolates were obtained and evaluated for salt stress tolerance at varying NaCl concentrations (up to 15%). All the studied isolates showed tolerance up to 7.5% NaCl concentration, only 3 isolates showed growth up to 12.5% and none of the isolates showed growth at 15 % NaCl concentration. Molecular identification using 16S rRNA gene sequencing revealed the predominance of genera such as Streptomyces spp. The N- fixation through acetylene reduction assay was in the range 0.22-0.87 µmoles ethylene/hr, all the isolates showed the ammonia and siderophore production, two isolate showed phosphorus solubilization, eight and ten isolates showed HCN (0.09-0.44), and exopolysaccharide production respectively. The IAA production 1.03-60.88 µg/ml and 1.41-32.45 µg/ml in presence and absence of tryptophan respectively. There was no significance difference was observed among the endophytes in ACC deaminase activity. These findings suggest that endo-rhizospheric actinobacteria possess dual functionality as salt stress alleviators and biofertilizers, emphasizing their potential contribution to sustainable agriculture in saline-affected areas.
Baker AW., & Schippers B. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology and Biochemistry. (1987);19:451-457. https://doi.org/10.1016/0038-0717(87)90037-X
Beneduzi A, Peres D, da Costa PB, Zanettini MHB, Passaglia LMP. Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil. Research in Microbiology. 2008;159(4):244-250. https://doi.org/10.1016/j.resmic.2008.03.003
Bhosale HJ and Kadam TA. Generic diversity and a comparative account on plant growth promoting characteristics of actinomycetes in roots and rhizosphere of Saccharum officinarum. Int J Curr Microbiol Appl Sci. 2015;4(1):230-244.
Bianco C, Defez R. Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain. J Exp Bot. 2009;60(11):3097-3107. https://doi.org/10.1093/jxb/erp140
Boubekri K, Soumare A, Mardad I, Lyamlouli K, Hafidi M, Ouhdouch Y, Kouisni L. The screening of potassium-and phosphate-solubilizing actinobacteria and the assessment of their ability to promote wheat growth parameters. Microorganisms. 2021;9(3):470. https://doi.org/10.3390/microorganisms9030470
Chantre Nongpiur R, Singla-Pareek SL, Pareek A. Genomics approaches for improving salinity stress tolerance in crop plants. Curr Genomics. 2016;17(4):343-357. https://doi.org/10.2174/1389202917666160331202517
Chukwuneme CF, Babalola OO, Kutu FR, Ojuederie OB. Characterization of actinomycetes isolates for plant growth promoting traits and their effects on drought tolerance in maize. J Plant Interact. 2020;15(1):93-105. https://doi.org/10.1080/17429145.2020.1752833
Coombs JT, Franco CM. Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol. 2003;69(9):5603-5608. https://doi.org/10.1128/aem.69.9.5603-5608.2003
Dahal B, NandaKafle G, Perkins L, Brözel VS. Diversity of free-living nitrogen fixing Streptomyces in soils of the badlands of South Dakota. Microbiol Res. 2017;195:31-39. https://doi.org/10.1016/j.micres.2016.11.004
Djebaili R, Pellegrini M, Rossi M, Forni C, Smati M, Del Gallo M, Kitouni M. Characterization of plant growth-promoting traits and inoculation effects on Triticum durum of actinomycetes isolates under salt stress conditions. Soil Syst. 2021;5(2):26. https://doi.org/10.3390/soilsystems5020026
Dubey A, Saiyam D, Kumar A, Hashem A, Abd_Allah EF, Khan ML. Bacterial root endophytes: characterization of their competence and plant growth promotion in soybean (Glycine max (L.) Merr.) under drought stress. Int J Environ Res Public Health. 2021;18(3):931. https://doi.org/10.3390/ijerph18030931
Egamberdieva D. Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiol Plant. 2009;31:861-864. http://dx.doi.org/10.1007/s11738-009-0297-0
Gangwar M, Rani S, Sharma N. Diversity of endophytic Actinomycetes from wheat and its potential as plant growth promoting and biocontrol agents. J Adv Lab Res Biol. 2012;3(1):18-23.
Gong Y, Chen LJ, Pan SY, Li XW, Xu MJ, Zhang CM, et al., Antifungal potential evaluation and alleviation of salt stress in tomato seedlings by a halotolerant plant growth-promoting actinomycete Streptomyces sp. KLBMP5084. Rhizosphere. 2020;16:100262. http://dx.doi.org/10.1016/j.rhisph.2020.100262
González F, Santander C, Ruiz A, Pérez R, Moreira J, Vidal G, et al., Inoculation with Actinobacteria spp. isolated from a hyper-arid environment enhances tolerance to salinity in lettuce plants (Lactuca sativa L.). Plants. 2023;12(10):2018. http://dx.doi.org/10.3390/plants12102018
Govindasamy, V., Senthilkumar, M., Gaikwad, K., Sharma, V., & Anandham, R. (2008). Identification and characterization of ACC deaminase gene (acdS) from plant growth-promoting Achromobacter xylosoxidans and Pseudomonas stutzeri. Current Microbiology, 57(4), 312–317. https://doi.org/10.1007/s00284-008-9203-3
Govindasamy V, Franco CM, Gupta VV. Endophytic actinobacteria: diversity and ecology. Adv Endophytic Res. 2014:27-59. http://dx.doi.org/10.1007/978-81-322-1575-2_2
Govindasamy V, George P, Raina SK, Kumar M, Rane J, Annapurna K. Plant-associated microbial interactions in the soil environment: role of endophytes in imparting abiotic stress tolerance to crops. Adv Crop Environ Interact. 2018:245-284. http://dx.doi.org/10.1007/978-981-13-1861-0_10
Hardy RW, Holsten RD, Jackson EK, Burns R. The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiol. 1968;43(8):1185-1207. https://doi.org/10.1104/pp.43.8.1185
Islam MR, Madhaiyan M, Boruah HPD, Yim WJ, Lee GS, Saravanan VS, et al., Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants. J Microbiol Biotechnol. 2009;19(10):1213-1222. https://doi.org/10.4014/jmb.0903.3028
Kaleem F, Shabir G, Aslam K, Rasul S, Manzoor H, Shah SM, Khan AR. An overview of the genetics of plant response to salt stress: present status and the way forward. Appl Biochem Biotechnol. 2018;186:306-334. https://doi.org/10.1007/s12010-018-2738-y
Kavya T, Govindasamy V, Suman A, Abraham G. Plant–Actinobacteria interactions for biotic and abiotic stress management in crops. In: Plant Holobiome Engineering for Climate-Smart Agriculture. Singapore: Springer Nature Singapore; 2024. p. 441-463. http://dx.doi.org/10.1007/978-981-99-9388-8_23
Khamna S, Yokota A, Peberdy JF, Lumyong S. Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. EurAsian J BioSci. 2010;4. http://dx.doi.org/10.5053/ejobios.2010.4.0.4
Kim TU, Cho SH, Han JH, Shin YM, Lee HB, Kim SB. Diversity and physiological properties of root endophytic actinobacteria in native herbaceous plants of Korea. J Microbiol. 2012;50:50-57. https://doi.org/10.1007/s12275-012-1417-x
Kruasuwan W, Thamchaipenet A. Diversity of culturable plant growth-promoting bacterial endophytes associated with sugarcane roots and their effect of growth by co-inoculation of diazotrophs and actinomycetes. J Plant Growth Regul. 2016;35:1074-1087. https://link.springer.com/article/10.1007/s00344-016-9604-3
Kumar S, Beena AS, Awana M, Singh A. Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Front Plant Sci. 2017;8:1151. https://doi.org/10.3389/fpls.2017.01151
Kumar V, Kumar A, Pandey KD, Roy BK. Isolation and characterization of bacterial endophytes from the roots of Cassia tora L. Ann Microbiol. 2015;65(3):1391-1399. https://doi.org/10.1007/s13213-014-0977-x
Lane DJ. 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics. 1991. p. 115-175.
Lee SO, Choi GJ, Choi YH, Jang KS, Park DJ, Kim CJ, Kim JC. Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae. J Microbiol Biotechnol. 2008;18(11):1741-1746. https://doi.org/10.4014/jmb.0800.108
Li Z, Chang S, Lin L, Li Y, An Q. A colorimetric assay of 1?aminocyclopropane?1?carboxylate (ACC) based on ninhydrin reaction for rapid screening of bacteria containing ACC deaminase. Lett Appl Microbiol. 2011;53(2):178-185. https://doi.org/10.1111/j.1472-765x.2011.03088.x
Mathew BT, Torky Y, Amin A, Mourad AHI, Ayyash MM, El-Keblawy A, et al., Halotolerant marine rhizosphere-competent actinobacteria promote Salicornia bigelovii growth and seed production using seawater irrigation. Front Microbiol. 2020;11:552. https://doi.org/10.3389/fmicb.2020.00552
Mingma R, Duangmal K, Mai Sci CJ. Characterization, antifungal activity and plant growth promoting potential of endophytic actinomycetes isolated from rice (Oryza sativa L.). Chiang Mai J Sci. 2018;45(7):2652-2665. http://it.science.cmu.ac.th/ejournal/
Mohamad OAA, Liu YH, Li L, Ma JB, Huang Y, Gao L, et al., Synergistic plant-microbe interactions between endophytic Actinobacteria and their role in plant growth promotion and biological control of cotton under salt stress. Microorganisms. 2022;10(5):867. https://doi.org/10.3390/microorganisms10050867
Munns R. Comparative physiology of salt and water stress. Plant Cell Environ. 2002;25(2):239-250. https://doi.org/10.1046/j.0016-8025.2001.00808.x
Nagrale DT, Chaurasia A, Kumar S, Gawande SP, Hiremani NS, Shankar R, et al., PGPR: the treasure of multifarious beneficial microorganisms for nutrient mobilization, pest biocontrol and plant growth promotion in field crops. World J Microbiol Biotechnol. 2023;39(4):100. https://doi.org/10.1007/s11274-023-03536-0
Narsing Rao MP, Lohmaneeratana K, Bunyoo C, Thamchaipenet A. Actinobacteria–plant interactions in alleviating abiotic stress. Plants. 2022;11(21):2976. https://doi.org/10.3390/plants11212976
Ndeddy Aka RJ, Babalola OO. Effect of bacterial inoculation of strains of Pseudomonas aeruginosa, Alcaligenes feacalis and Bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. Int J Phytoremediation. 2016;18(2):200-209. https://doi.org/10.1080/15226514.2015.1073671
Nozari RM, Ramos LM, da Luz LA, Almeida RN, Lucas AM, Cassel E, et al., Halotolerant Streptomyces spp. induce salt tolerance in maize through systemic induction of the antioxidant system and accumulation of proline. Rhizosphere. 2022;24:100623. http://dx.doi.org/10.1016/j.rhisph.2022.100623
Passari AK, Chandra P, Mishra VK, Leo VV, Gupta VK, Kumar B, Singh BP. Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant-growth-promoting effect. Res Microbiol. 2016;167(8):692-705. https://doi.org/10.1016/j.resmic.2016.07.001
Paul D and Lade H. Plant-growth-promoting rhizobacteria to improve crop growth in saline soils: a review. Agron Sustain Dev. 2014;34:737-752. https://doi.org/10.1007/s13593-014-0233-6
Rashid S, Charles TC, Glick BR. Isolation and characterization of new plant growth-promoting bacterial endophytes. Appl Soil Ecol. 2012;61:217-224. https://doi.org/10.1016/j.apsoil.2011.09.011
Rungin S, Indananda C, Suttiviriya P, Kruasuwan W, Jaemsaeng R, Thamchaipenet A. Plant growth enhancing effects by a siderophore-producing endophytic streptomycete isolated from a Thai jasmine rice plant (Oryza sativa L. cv. KDML105). Antonie Van Leeuwenhoek. 2012;102:463-472. https://doi.org/10.1007/s10482-012-9778-z
Sadeghi A, Karimi E, Dahaji PA, Javid MG, Dalvand Y, Askari H. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World J Microbiol Biotechnol. 2012;28:1503–1509. https://doi.org/10.1007/s11274-011-0952-7
Saikia K and Bora LC. Exploring actinomycetes and endophytes of rice ecosystem for induction of disease resistance against bacterial blight of rice. Eur J Plant Pathol. 2021;159:67-79.
Sánchez-Suárez J, Coy-Barrera E, Villamil L, Díaz L. Streptomyces-derived metabolites with potential photoprotective properties—a systematic literature review and meta-analysis on the reported chemodiversity. Molecules. 2020;25:3221. https://doi.org/10.3390/molecules25143221
Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987;160(1):47-56. https://doi.org/10.1016/0003-2697(87)90612-9
Sharma AK, Sommerfeld K, Bullerjahn GS, Matteson AR, Wilhelm SW, Jezbera J, et al., Actinorhodopsin genes discovered in diverse freshwater habitats and among cultivated freshwater Actinobacteria. ISME J. 2009;3(6):726-737. https://doi.org/10.1038/ismej.2009.13
Sreevidya M, Gopalakrishnan S, Kudapa H, Varshney RK. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Braz J Microbiol. 2016;47:85-95. https://doi.org/10.1016/j.bjm.2015.11.030
Sukpanoa S, Kaewkla O, Suriyachadkun C, Papayrata C, Klankeo P, Franco CMM. Streptomyces mahasarakhamensis sp. nov., an endophytic actinobacterium isolated from jasmine rice and its potential as plant growth promoter. Curr Microbiol. 2024;81(8):223. https://doi.org/10.1007/s00284-024-03747-0
Sylvester-Bradley R, Asakawa N, Torraca SL, Magalhães FM, Oliveira LA, Pereira RM. Levantamento quantitativo de microrganismos solubilizadores de fosfatos na rizosfera de gramíneas e leguminosas forrageiras na Amazônia. Acta Amazon. 1982;12(1):15-22. https://doi.org/10.1590/1809-43921982121015
Thokchom E, Thakuria D, Kalita MC, Sharma CK, Talukdar NC. Root colonization by host-specific rhizobacteria alters indigenous root endophyte and rhizosphere soil bacterial communities and promotes the growth of mandarin orange. Eur J Soil Biol. 2017;79:48-56.
Tian X, Cao L, Tan H, Han W, Chen M, Liu Y, Zhou S. Diversity of cultivated and uncultivated actinobacterial endophytes in the stems and roots of rice. Microb Ecol. 2007;53:700-707. https://doi.org/10.1007/s00248-006-9163-4
Verhoef RP, Schols HA, Voragen AGJ. (2003) Exopolysaccharides produced by bacteria isolated from process water environment. [Journal info incomplete].
Verma VC, Singh SK, Prakash S. Bio?control and plant growth promotion potential of siderophore producing endophytic Streptomyces from Azadirachta indica A. Juss. J Basic Microbiol. 2011;51(5):550-556. https://doi.org/10.1002/jobm.201000155
Xiong YW, Gong Y, Li XW, Chen P, Ju XY, Zhang CM, et al., Enhancement of growth and salt tolerance of tomato seedlings by a natural halotolerant actinobacterium Glutamicibacter halophytocola KLBMP 5180 isolated from a coastal halophyte. Plant Soil. 2019;445:307-322. https://link.springer.com/article/10.1007/s11104-019-04310-8
Yandigeri MS, Meena KK, Singh D, Malviya N, Singh DP, Solanki MK, et al., Drought-tolerant endophytic actinobacteria promote growth of wheat (Triticum aestivum) under water stress conditions. Plant Growth Regul. 2012;68:411-420. http://dx.doi.org/10.1007/s10725-012-9730-2
Zahra ST, Tariq M, Abdullah M, Zafar M, Yasmeen T, Shahid MS, et al., Probing the potential of salinity-tolerant endophytic bacteria to improve the growth of mungbean [Vigna radiata (L.) Wilczek]. Front Microbiol. 2023;14:1149004. https://doi.org/10.3389/fmicb.2023.1149004
Zahra T, Hamedi J, Mahdigholi K. Endophytic actinobacteria of a halophytic desert plant Pteropyrum olivieri: promising growth enhancers of sunflower. 3 Biotech. 2020;10(12):514. https://doi.org/10.1007/s13205-020-02507-8
Zaman M, Shahid SA, Heng L, Shahid SA, Zaman M, Heng L. Soil salinity: Historical perspectives and a world overview of the problem. Guideline for salinity assessment, mitigation and adaptation using nuclear and related techniques. 2018:43-53. https://doi.org/10.1007/978-3-319-96190-3_2
Zhao K, Penttinen P, Guan T, Xiao J, Chen Q, Xu J, et al., The diversity and antimicrobial activity of endophytic actinomycetes isolated from medicinal plants in Panxi plateau, China. Curr Microbiol. 2011;62:182-190. https://doi.org/10.1007/s00284-010-9685-3
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