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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study, medium components and production conditions were different for carbon and nitrogen sources. The results have shown that the best carbon and nitrogen sources were lactose and yeast extract at concentration 1%. The different physiological factors include Temperature, pH, aeration and agitation. Besides, the results showed that the maximum production of L-Asparaginase was achieved after the incubation of the growth culture at 30°C and pH 7.Under these conditions, the activity of L-Asparaginase produced in culture medium was (10.3 IU/ml) and (10.5 IU/ml) respectively. The production of L-Asparaginase was modified during E.coli grown aerobically and when the shaker incubated at 220 rpm.