National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Among 33 isolates three potent isolates viz. M 3, M 10 and M 15 showed fourfold increase in cell numbers in methanol 1% solution. Isolate M 3, M 10 and M 15 were found Gram +ve, rods occurring singly. Isolate M 3, M 10 and M 15 showed yellow, dull white and shiny white pigmented growth respectively, on NMS media. From cultural and microscopic characteristics it was accepted that, isolates belongs to family Bacillaceae. Isolate M 15 were found tolerating higher pH range (9.0) and 7.5% NaCl concentration as compared to other isolates Isolate M 10 and M 15 showed resistant to wide range of antibiotics and were found to utilize carbon sources. On the basis of partial 16S rRNA gene sequencing selected phyllospheric isolates, AAU M 3 was identified as Staphylococcus saprophyticus, AAU M 10 as Bacillus subtilis, AAU M 15 as Bacillus methylotrophicus. Chosen three isolates were subjected to qualitative detection of two key enzymes of bacterial methane degradation pathway viz. soluble methane monooxygenase (sMMO) and methanol dehydrogenase (MDH) and established positive.
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