National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this present study, 27 rice-rhizosphere actinobacterial isolates were screened for salt (NaCl) stress tolerance and plant growth-promoting (PGP) traits. Significant growth was observed in 24, 12, and three isolates at 7.5%, 10%, and 12.5% NaCl, respectively. Quantitative screening revealed nitrogen fixation in 13 isolates (0.65±0.04 to 28.68±0.58 µmoles ethylene/hr), phosphorus solubilization in six isolates, and siderophore, HCN, and exopolysaccharide production in 20, 25, and 16 isolates, respectively. The phytohormone, auxins (IAA) production was varied from 96.05±0.03 to 4.72±0.05 µg/ml and 90.58±0.02 to 7.52±0.01 µg/ml in the presence (19 isolates) and absence (20 isolates) of precursor L-tryptophan, respectively. Among the 18 actinobacterial isolates identified, ACC deaminase enzyme activity was reported in terms of ACC consumption in the range of 2.41±1.56 to 3.07±0.97 mmol. Among the 27-actinobactreial isolates, molecular identification of 8 isolates revealed them they are belonging to different species of actinobacterial genus Streptomyces. Based on the results of NaCl tolerance and PGP traits screening, three rice-rhizosphere actinobacterial isolates were selected as promising salt stress tolerant and possessing multiple-PGP traits, which were phylogenetically identified as Kitasatospora sp. strain Met24, Streptomyces tritolerans strain V04a and Streptomyces sp. strain Humic 11a. The halotolerant rice-rhizosphere actinobacteria identified and characterized in this study could be a promising candidate for utilization as growth stimulating bioinoculants subject to their further evaluation/investigations in the rice crop under salt stress conditions.
Adam, D., Maciejewska, M., Naômé, A., Martinet, L., Coppieters, W., Karim, L.,... & Rigali, S. (2018). Isolation, characterization, and antibacterial activity of hard-to-culture actinobacteria from cave moonmilk deposits. Antibiotics, 7(2), 28. https://doi.org/10.3390/antibiotics7020028
Afridi, M. S., Mahmood, T., Salam, A., Mukhtar, T., Mehmood, S., Ali, J.,... & Chaudhary, H. J. (2019). Induction of tolerance to salinity in wheat genotypes by plant growth promoting endophytes: Involvement of ACC deaminase and antioxidant enzymes. Plant Physiology and Biochemistry, 139, 569-577. https://doi.org/10.1016/j.plaphy.2019.04.007
Afridi, M. S., Mahmood, T., Salam, A., Mukhtar, T., Mehmood, S., Ali, J.,... & Chaudhary, H. J. (2019). Induction of tolerance to salinity in wheat genotypes by plant growth promoting endophytes: Involvement of ACC deaminase and antioxidant enzymes. Plant Physiology and Biochemistry, 139, 569-577.
Ahmed, E., & Holmström, S. J. (2014). Siderophores in environmental research: roles and applications. Microbial biotechnology, 7(3), 196-208. https://doi.org/10.1111/1751-7915.12117
Akond, M. A., Jahan, M. N., Sultana, N., & Rahman, F. (2016). Effect of temperature, pH and NaCl on the isolates of actinomycetes from straw and compost samples from Savar, Dhaka, Bangladesh. American Journal of Microbiology and Immunology, 1(2), 10-15.
Baker, A. W., & Schippers, B. (1987). Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology and Biochemistry, 19, 451-457. https://doi.org/10.1016/0038-0717(87)90037-X
Beneduzi, A., Peres, D., da Costa, P. B., Zanettini, M. H. B., & Passaglia, L. M. P. (2008). Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil. Research in Microbiology, 159(4), 244-250. https://doi.org/10.1016/j.resmic.2008.02.001
Bhise, K. K., & Dandge, P. B. (2019). Mitigation of salinity stress in plants using plant growth promoting bacteria. Symbiosis, 79(3), 191-204. https://doi.org/10.1007/s13199-019-00642-3
Chen, S. M., Zhang, C. M., Peng, H., Qin, Y. Y., Li, L., Li, C. G.,... & Qin, S. (2023). Exopolysaccharides from endophytic Glutamicibacter halophytocota KLBMP 5180 functions as bio-stimulants to improve tomato plants growth and salt stress tolerance. International Journal of Biological Macromolecules, 253, 126717. https://doi.org/10.1016/j.ijbiomac.2023.126717
Chouyia, F. E., Romano, I., Fechtali, T., Fagnano, M., Fiorentino, N., Visconti, D.,... & Pepe, O. (2020). P-solubilizing Streptomyces roseocinereus MS1B15 with multiple plant growth-promoting traits enhance barley development and regulate rhizosphere microbial population. Frontiers in Plant Science, 11, 1137. https://doi.org/10.3389/fpls.2020.01137
Chukwuneme, C. F., Babalola, O. O., Kutu, F. R., & Ojuederie, O. B. (2020). Characterization of actinomycetes isolates for plant growth promoting traits and their effects on drought tolerance in maize. Journal of Plant Interactions, 15(1), 93-105. https://doi.org/10.1080/17429145.2020.1730243
Coombs, J. T., & Franco, C. M. (2003). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Applied and environmental microbiology, 69(9), 5603-5608. https://doi.org/10.1128/AEM.69.9.5603-5608.2003
Dahal, B., NandaKafle, G., Perkins, L., & Brözel, V. S. (2017). Diversity of free-Living nitrogen fixing Streptomyces in soils of the badlands of South Dakota. Microbiological Research, 195, 31-39. https://doi.org/10.1016/j.micres.2016.11.001
del Carmen Orozco-Mosqueda, M., Glick, B. R., & Santoyo, G. (2020). ACC deaminase in plant growth-promoting bacteria (PGPB): An efficient mechanism to counter salt stress in crops. Microbiological Research, 235, 126439. https://doi.org/10.1016/j.micres.2019.126439
Djebaili, R., Pellegrini, M., Rossi, M., Forni, C., Smati, M., Del Gallo, M., & Kitouni, M. (2021). Characterization of plant growth-promoting traits and inoculation effects on Triticum durum of actinomycetes isolates under salt stress conditions. Soil systems, 5(2), 26. https://doi.org/10.3390/soilsystems5020026
Elshahat, M. R., Ahmed, A. A., Enas, A. H., & Fekria, M. S. (2016). Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens. African Journal of Microbiology Research, 10(15), 486-504.
El?Tarabily, K. A., Nassar, A. H., Hardy, G. S. J., & Sivasithamparam, K. (2009). Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes. Journal of Applied Microbiology, 106(1), 13-26. https://doi.org/10.1111/j.1365-2672.2008.03959.x
El-Tarabily, K. A., Sham, A., Elbadawi, A. A., Hassan, A. H., Alhosani, B. K., El-Esawi, M. A.,... & AbuQamar, S. F. (2021). A consortium of rhizosphere-competent actinobacteria exhibiting multiple plant growth-promoting traits improves the growth of Avicennia marina in the United Arab Emirates. Frontiers in Marine Science, 8, 715123. https://doi.org/10.3389/fmars.2021.715123
Empadinhas, N., & da Costa, M. S. (2008). Osmoadaptation mechanisms in prokaryotes: distribution of compatible solutes. Int Microbiol, 11(3), 151-61.
Fitri, L., Bessania, M. A., Septi, N., & Suhartono, S. (2021). Isolation and characterization of soil actinobacteria as cellulolytic enzyme producer from Aceh Besar, Indonesia. Biodiversitas Journal of Biological Diversity, 22(11). https://doi.org/10.13057/biodiv/d221131
Flowers, T. J., & Yeo, A. R. (1981). Variability in the resistance of sodium chloride salinity within rice (Oryza sativa L.) varieties. New Phytologist, 88(2), 363-373. https://doi.org/10.1111/j.1469-8137.1981.tb01716.x
Franco-Correa, M., Quintana, A., Duque, C., Suarez, C., Rodríguez, M. X., & Barea, J. M. (2010). Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Applied Soil Ecology, 45(3), 209-217. https://doi.org/10.1016/j.apsoil.2010.04.007
Gao, Y., Han, Y., Li, X., Li, M., Wang, C., Li, Z.,... & Wang, W. (2022). A salt-tolerant Streptomyces paradoxus D2-8 from rhizosphere soil of Phragmites communis augments soybean tolerance to soda saline-alkali stress. Polish journal of microbiology, 71(1), 43. https://doi.org/10.33073/pjm-2022-005
Gong, Y., Bai, J. L., Yang, H. T., Zhang, W. D., Xiong, Y. W., Ding, P., & Qin, S. (2018). Phylogenetic diversity and investigation of plant growth-promoting traits of actinobacteria in coastal salt marsh plant rhizospheres from Jiangsu, China. Systematic and applied microbiology, 41(5), 516-527. https://doi.org/10.1016/j.syapm.2018.04.005
González, F., Santander, C., Ruiz, A., Pérez, R., Moreira, J., Vidal, G.,... & Cornejo, P. (2023). inoculation with Actinobacteria spp. isolated from a hyper-arid environment enhances tolerance to salinity in lettuce plants (Lactuca sativa L.). Plants, 12(10), 2018. https://doi.org/10.3390/plants12102018
Govindasamy, V., Raina, S. K., George, P., Kumar, M., Rane, J., Minhas, P. S., & Vittal, K. P. R. (2017). Functional and phylogenetic diversity of cultivable rhizobacterial endophytes of sorghum [Sorghum bicolor (L.) Moench]. Antonie van Leeuwenhoek, 110(7), 925–943. https://doi.org/10.1007/s10482-017-0862-2
Grayston, R., D. Vaughan and D. Jones, 1996. Rhizosphere carbon flow in trees, in comparison with annual plants: the importance of root exudation and its impact on microbial activity and nutrient availability. Appl. Soil Ecology, 5(1): 29-56. https://doi.org/10.1016/S0929-1393(96)00126-6
Hardy, R. W., Holsten, R. D., Jackson, E. K., & Burns, R. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant physiology, 43(8), 1185-1207. https://doi.org/10.1104/pp.43.8.1185
Hu, Y., Qiu, L., Zhang, Z., Liu, K., Xia, X., Xiong, S.,... & Liang, Y. (2021). Control of Streptomyces alfalfae XY25 T over clubroot disease and its effect on rhizosphere microbial community in Chinese cabbage field trials. Frontiers in microbiology, 12, 641556. https://doi.org/10.3389/fmicb.2021.641556
Islam, M. R., Madhaiyan, M., Boruah, H. P. D., Yim, W. J., Lee, G. S., Saravanan, V. S.,... & Sa, T. (2009). Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants. Journal of Microbiology and Biotechnology, 19(10), 1213-1222. https://doi.org/10.4014/jmb.0901.0007
Jiang, Y., Cao, Y. R., Wiese, J., Tang, S. K., Xu, L. H., Imhoff, J. F., & Jiang, C. L. (2011). Streptomyces sparsus sp. nov., isolated from a saline and alkaline soil. International journal of systematic and evolutionary microbiology, 61(7), 1601-1605. https://doi.org/10.1099/ijs.0.024620-0
Kavya, T., Govindasamy, V., Suman, A., & Abraham, G. (2024). Plant–Actinobacteria Interactions for Biotic and Abiotic Stress Management in Crops. In Plant Holobiome Engineering for Climate-Smart Agriculture (pp. 441-463). Singapore: Springer Nature Singapore.
Kumawat, K. C., Nagpal, S., & Sharma, P. (2022). Potential of plant growth-promoting rhizobacteria-plant interactions in mitigating salt stress for sustainable agriculture: A review. Pedosphere, 32(2), 223-245. https://doi.org/10.1016/S1002-0160(21)60072-5
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics, 115-175.
Li, Z., Chang, S., Lin, L., Li, Y., & An, Q. (2011). A colorimetric assay of 1?aminocyclopropane?1?carboxylate (ACC) based on ninhydrin reaction for rapid screening of bacteria containing ACC deaminase. Letters in Applied Microbiology, 53(2), 178-185. https://doi.org/10.1111/j.1472-765X.2011.03134.x
Liu, X.; Chai, J.; Zhang, Y.; Zhang, C.; Lei, Y.; Li, Q.; Yao, T. Halotolerant rhizobacteria mitigate the effects of salinity stress on maize growth by secreting exopolysaccharides. Environ. Exp. Bot. 2022, 204, 105098. https://doi.org/10.1016/j.envexpbot.2022.105098
Malisorn, K., Embaen, S., Sribun, A., Saeng-In, P., Phongsopitanun, W., & Tanasupawat, S. (2020). Identification and antimicrobial activities of Streptomyces, Micromonospora, and Kitasatospora strains from rhizosphere soils. Journal of Applied Pharmaceutical Science, 10(2), 123-128 https://doi.org/10.7324/JAPS.2020.102016
Meena, K. K., Bitla, U. M., Sorty, A. M., Singh, D. P., Gupta, V. K., Wakchaure, G. C., & Kumar, S. (2020). Mitigation of salinity stress in wheat seedlings due to the application of phytohormone-rich culture filtrate extract of methylotrophic actinobacterium Nocardioides sp. NIMMe6. Frontiers in Microbiology, 11, 2091. https://doi.org/10.3389/fmicb.2020.02091
Mohamed, H. I., & Gomaa, E. Z. (2012). Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress. Photosynthetica, 50, 263-272. https://doi.org/10.1007/s11099-012-0032-5
Mohamed, H., Miloud, B., Zohra, F., José María García-Arenzana, J. M., Veloso, A., & Rodríguez-Couto, S. (2017). Isolation and characterisation of Actinobacteria from Algerian sahara soils with antimicrobial activities. International Journal of Molecular and Cellular Medicine (IJMCM), 6(2), 109-120.
Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911
Ndeddy Aka, R. J., & Babalola, O. O. (2016). Effect of bacterial inoculation of strains of Pseudomonas aeruginosa, Alcaligenes feacalis and Bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. International Journal of Phytoremediation, 18(2), 200-209. https://doi.org/10.1080/15226514.2015.1073671
Nozari, R. M., Ortolan, F., Astarita, L. V., & Santarém, E. R. (2021). Streptomyces spp. enhance vegetative growth of maize plants under saline stress. Brazilian Journal of Microbiology, 52(3), 1371-1383. https://doi.org/10.1007/s42770-021-00477-4
Priya, E., Nagasathya, A., Steffi, P. F., & Thamilmaraiselvi, B. (2020). Characterization of Actinobacteria Isolated from Saltpan, Environment of Thondi, Ramanathapuram Dt. Research Journal of Pharmacy and Technology, 13(11), 5108-5114.
Romano-Armada, N., Yañez-Yazlle, M. F., Irazusta, V. P., Rajal, V. B., & Moraga, N. B. (2020). Potential of bioremediation and PGP traits in Streptomyces as strategies for bio-reclamation of salt-affected soils for agriculture. Pathogens, 9(2), 117. https://doi.org/10.3390/pathogens9020117
Sadeghi, A., Karimi, E., Dahaji, P. A., Javid, M. G., Dalvand, Y., & Askari, H. (2012). Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology, 28, 1503-1509. https://doi.org/10.1007/s11274-011-0941-7
Schwyn, B., & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry, 160(1), 47-56. https://doi.org/10.1016/0003-2697(87)90612-9
Sreevidya, M., Gopalakrishnan, S., Kudapa, H., & Varshney, R. K. (2016). Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. brazilian journal of microbiology, 47, 85-95. https://doi.org/10.1016/j.bjm.2015.11.011
Sylvester-Bradley, R., Asakawa, N., Torraca, S. L., Magalhães, F. M., Oliveira, L. A., & Pereira, R. M. (1982). Levantamento quantitativo de microrganismos solubilizadores de fosfatos na rizosfera de gramíneas e leguminosas forrageiras na Amazônia. Acta Amazonica, 12(1), 15-22.
Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular biology and evolution, 24(8), 1596-1599. https://doi.org/10.1093/molbev/msm092
Thumar, J. T., & Singh, S. P. (2009). Organic solvent tolerance of an alkaline protease from salt-tolerant alkaliphilic Streptomyces clavuligerus strain Mit-1. Journal of Industrial Microbiology and Biotechnology, 36(2), 211. https://doi.org/10.1007/s10295-008-0497-6
Ting, A. S. Y., Tan, S. H., & Wai, M. K. (2009). Isolation and characterization of Actinobacteria with antibacterial activity from soil and rhizosphere soil. Australian Journal of Basic and Applied Sciences, 3(4), 4053-4059.
Valdés, María, Néstor-Octavio Pérez, Paulina Estrada-de Los Santos, Jesús Caballero-Mellado, Juan José Peña-Cabriales, Philippe Normand, and Ann M. Hirsch. "Non-Frankia actinomycetes isolated from surface-sterilized roots of Casuarina equisetifolia fix nitrogen." Applied and environmental microbiology 71, no. 1 (2005): 460-466. https://doi.org/10.1128/AEM.71.1.460-466.2005
Verhoef, R. P., Schols, H. A., & Voragen, A. G. J. (2003). Exopolysaccharides produced by bacteria isolated from process water environment.
Vijayabharathi, R., Sathya, A., & Gopalakrishnan, S. (2016). A renaissance in plant growth-promoting and biocontrol agents by endophytes. Microbial Inoculants in Sustainable Agricultural Productivity: Vol. 1: Research Perspectives, 37-60.
Wang, W., Qiu, Z., Tan, H., & Cao, L. (2014). Siderophore production by actinobacteria. Biometals, 27, 623-631. https://doi.org/10.1007/s10534-014-9742-3
Yoolong, S., Kruasuwan, W., Thanh Ph?m, H. T., Jaemsaeng, R., Jantasuriyarat, C., & Thamchaipenet, A. (2019). Modulation of salt tolerance in Thai jasmine rice (Oryza sativa L. cv. KDML105) by Streptomyces venezuelae ATCC 10712 expressing ACC deaminase. Scientific Reports, 9(1), 1275. https://doi.org/10.1038/s41598-018-38247-5
Yasmeen, T., Ahmad, A., Arif, M. S., Mubin, M., Rehman, K., Shahzad, S. M., Iqbal, S., Rizwan, M., Ali, S., Alyemeni, M. N., & Wijaya, L. (2020). Biofilm forming rhizobacteria enhance growth and salt tolerance in sunflower plants by stimulating antioxidant enzyme activity. Plant Physiology and Biochemistry, 156, 242–256. https://doi.org/10.1016/j.plaphy.2020.09.016
Citations |
 |
 |
 |
 |
