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NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2023: 95.56
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
For more details click here
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Original Research Articles                      Volume : 15, Issue:3, March, 2026
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2026.15(3): 149-158
DOI: https://doi.org/10.20546/ijcmas.2026.1503.014


Standardization of Infrared Pre-treatment Process for Detoxification of Cottonseed Meal and Enhancement of Protein Extraction
Shalaka Kalamnurikar1*, Smita Khodke1 and Dattatreya Kadam2
1Department of Processing and Food Engineering CAET, VNMKV, Parbhani. (MS), India 3Principal Scientist and Head ICAR-Central Institute for Research on Cotton Technology, Mumbai. (MS), India
*Corresponding author
Abstract:

Cottonseed meal (CSM) is a protein-rich by-product whose utilization is restricted by the presence of toxic free gossypol. This study optimized infrared (IR) pre-treatment conditions to reduce free gossypol while preserving protein quality for efficient extraction. CSM was treated at 50–120 °C for 1–5 min under controlled conditions and analyzed for free gossypol, total gossypol, and protein content. Free gossypol decreased progressively up to 70 °C, reaching a minimum of 0.036 % at 70 °C for 5 min, but increased at higher temperatures (0.111 % at 120 °C), indicating disruption of protein–gossypol complexes. Total gossypol slightly decreased up to 70 °C (≈1.21 %) and increased thereafter (≈2.01 % at 120 °C). Protein content improved significantly at moderate temperatures, with maximum extractable protein (≈60–65 %) at 70 °C for 2–3 min, whereas severe heating (≥90 °C) caused substantial protein denaturation. Overall, IR pre-treatment at 70–80 °C for 2–5 min was identified as optimal for effective detoxification while maintaining protein integrity, offering a rapid and scalable strategy for cottonseed protein isolation and value-added applications.


Keywords: Cottonseed meal, Gossypol detoxification, Infrared pre-treatment, Protein extraction

References:
  1. Kotecka-Majchrzak, K., Sumara, A., & Fornal, E. (2020). Oilseed by-products as sources of plant protein for human nutrition. Trends in Food Science & Technology, 106, 160–170. https://doi.org/1016/j.tifs.2020.10.004
  2. Vinayashree S, Vasu P. (2021). Biochemical, nutritional and functional properties of protein isolate and fractions from pumpkin (Cucurbita moschata var. Kashi Harit) seeds. Food Chemistry, 340:128177. https://doi.org/1016/j.foodchem.2020.128177
  3. Poji?, M., Mišan, A., & Tiwari, B. K. (2018). Eco-innovative technologies for extraction of proteins from plant by-products. Trends in Food Science & Technology, 75, 93–104. https://doi.org/1016/j.tifs.2018.03.010
  4. Kumar, M., Potkule, J., Patil, S., Saxena, S., Patil, P. G., Mageshwaran, V., Punia, S., Varghese, E., Mahapatra, A., Ashtaputre, N. & Charlene, D. S. (2021). Extraction of ultra-low gossypol protein from cottonseed: Characterization based on antioxidant activity, structural morphology and functional group analysis. Lwt. 140, 110692. https://doi.org/1016/j.lwt.2020.110692
  5. Yuliarti, O., Kiat Kovis, T. J., & Yi, N. J. (2021). Structuring the meat analogue by using plant-based derived composites. Journal of Food Engineering, 288, 110138. https://doi.org/1016/j.jfoodeng.2020.110138
  6. Sá, A. G. A., Moreno, Y. M. F., & Carciofi, B. A. M. (2020). Plant proteins as emerging sustainable sources for food applications: A review. Trends in Food Science & Technology, 97, 315–328 https://doi.org/1016/j.tifs.2020.02.007
  7. He, Z., Zhang, D., & Cao, H. (2018). Protein profiling of water and alkali soluble cottonseed protein isolates. Scientific Reports, 8(1). https://doi.org/1038/s41598-018-31113-3
  8. Mageshwaran, V., D’souza, C., Kambli, N., & Kathe, A.A. (2015). Fermentation technology: A viable tool for value-addition to cotton byproducts. Cotton Research Journal, 7(1), 54–60
  9. Food and Drug Administration (FDA). 1974. Food Drug Cosmetic Law; Food and Drug Administration: Silver Spring, MD, USA.
  10. Mageshwaran, V. (2021). An overview of gossypol and methods of its detoxification in cottonseed meal for non-ruminant feed applications. Indian Journal of Natural Products and Resources.12(3), 348-358.
  11. Romero, A. D. C., Calori-Domingues, M. A., Abdalla, A. L. & Augusto, P. E. D. (2021). Evaluation of ozone technology as an alternative for degradation of free gossypol in cottonseed meal: a prospective study. Food Additives & Contaminants: Part A. 38(4), 659-669. https://doi.org/1080/19440049.2020.1869734
  12. Satankar, V., Singh, M., Mageshwaran, V., Jhodkar, D., Changan, S., Kumar, M. & Mekhemar, M. (2021). Cottonseed kernel powder as a natural health supplement: An approach to reduce the gossypol content and maximize the nutritional benefits. Applied Sciences. 11(9), 3901. https://doi.org/3390/app11093901
  13. European Food Safety Authority (EFSA) Scientific Opinion-Gossypol as an undesirable substance in animal feed. (2008). Scientific opinion of the panel on contaminants in the food chain (Question No EFSA-Q-2005-222). EFSA J. 955:1–55 https://doi.org/2903/j.efsa.2008.908
  14. Gerasimidis, K., Fillou, D. T., Babatzimcpoulou, M., Tassou, K. & Katsikas, H. (2007). Preparation of an edible cottonseed protein concentrate and evaluation of its functional properties. International Journal of Food Sciences and Nutrition, 58(6), 486-490. https://doi.org/1080/09637480701343715
  15. Wang, X., Howell, C. P., Chen, F., Yin, J. & Jiang, Y. (2009). Gossypol-a polyphenolic compound from cotton plant. Advances in Food and Nutrition Research, 58, 215-263. https://doi.org/1016/S1043-4526(09)58008-3
  16. Shawrang, P., Mansouri, M. H., Sadeghi, A. A. & Ziaie, F. (2011). Evaluation and comparison of gamma-and electron beam irradiation effects on total and free gossypol of cottonseed meal. Radiation Physics and Chemistry. 80(6), 761-762. https://doi.org/1016/j.radphyschem.2011.01.011
  17. Hahm, S. W., Son, H., Kim, W., Oh, Y. K. & Son, Y. S. (2013). Effects of gamma irradiation on nutrient composition, anti-nutritional factors, in vitro digestibility and ruminal degradation of whole cotton seed. Journal of Animal Science and Technology. 55(2), 123-130. https://doi.org/5187/JAST.2013.55.2.123
  18. Nayefi, M., Salari, S., Sari, M. & Behgar, M. (2014). Treatment by gamma or electron radiation decreases cell wall and gossypol content of cottonseed meal. Radiation Physics and Chemistry. 99, 23-25. https://doi.org/10.1016/j.radphyschem.2014.02.008
  1. Maetens, E., Hettiarachchy, N., Dewettinck, K., Horax, R., & Moens, K. (2018). Reductions of anti-nutritional factors of germinated soybeans by ultraviolet and infrared treatments for snack chips preparation. LWT – Food Science and Technology, 90, 513–518. https://doi.org/10.1016/j.lwt.2017.12.032
  1. Collison, M. W. (2017). AOCS, Official methods and recommended Practices of the AOCS, (7th ), 2017, pp.Ba 8-78.
  2. George, W. & Latimer, J. R. (2019). Official method of analysis of AOAC” by AOAC international 21st Edition.
  3. Zhang, Z., Yang, D., Liu, L., Chang, Z., & Peng, N. (2022). Effective gossypol removal from cottonseed meal through optimized solid-state fermentation by Bacillus coagulans. Microbial Cell Factories, 21(1). https://doi.org/1186/s12934-022-01905-2
  4. Limpisophon, K., Maqsood, S., & Benjakul, S. (2024). Thermal modification of food proteins: Structural changes and functional implications. Trends in Food Science & Technology, 146, 104362. https://doi.org/10.1016/j.tifs.2024.104362
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How to cite this article:

Shalaka Kalamnurikar, Smita Khodke and Dattatreya Kadam. 2026. Standardization of Infrared Pre-treatment Process for Detoxification of Cottonseed Meal and Enhancement of Protein Extraction.Int.J.Curr.Microbiol.App.Sci. 15(3): 149-158. doi: https://doi.org/10.20546/ijcmas.2026.1503.014
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