National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Skin infections have always been a cause of concern for many years now, especially, infections caused by wounds. The current strategies largely involve use of antibiotics which add on to the current problem of antimicrobial resistance. Therefore, using biocompatible materials and bioactive molecules of probiotics can be used to treat the skin infections and to ensure skin care. This study explores the potential of bacteriocin-like substances extracted from probiotic strains namely, Lactobacillus rhamnosus LGG and Lactobacillus plantarum and isolate (IS1) obtained from commercial Kefir starter culture powder. The preliminary identification of the IS 1 revealed to belong to Lactobacillus strain (Lactobacillus ferintoshensis). Further, antioxidant potential of the three probiotic strains revealed that Lactobacillus rhamnosus possess the most antioxidant potential followed by Lactobacillus plantarum. Bacteriocins extracted from these strains demonstrated antimicrobial and antibiofilm activity against E. coli, S. aureus, and P. aeruginosa. These bacteriocins were successfully incorporated into a biocompatible pectin–chitosan hydrogel matrix formulated using pectin extracted from dried orange peels. The developed gel showed antimicrobial activity, indicating effective diffusion of bacteriocins from the matrix
Ahirwar, S. S., Gupta, M. K., Gupta, G., & Singh, V. (2017). Screening, isolation and identification of Lactobacillus species from dental caries of children. International Journal of Current Microbiology and Applied Sciences, 6(1), 497–503. https://doi.org/10.20546/ijcmas.2017.601.059
Al-Attwani, J. H. (2014). The effect of probiotics on bacterial human skin pathogens (Doctoral dissertation, Plymouth University, United Kingdom). Plymouth University.
Alamineh, E. A. (2018). Extraction of pectin from orange peels and characterizing its physical and chemical properties. American Journal of Applied Chemistry, 6(2), 51–56. https://doi.org/10.11648/j.ajac.20180602.13
Arshad, T., Mundrathi, V., Perez, V. E., Nunez, J. M., & Cho, H. (2024). Topical probiotic hydrogels for burn wound healing. Gels, 10(9), 545. https://doi.org/10.3390/gels10090545
Azimi, S. G., Moosavi, F. M., Khoshbin, Z., Shakour, N., & Kazemi, S. (2025). Synthesis and swelling studies of modified chitosan smart hydrogels containing alkyl sulfonate anionic pendant groups as microparticles for insulin release. Scientific Reports, 15(1), 26166. https://doi.org/10.1038/s41598-025-06494-9
Batoni, G., Catelli, E., Kaya, E., Pompilio, A., Bianchi, M., Ghelardi, E., Di Bonaventura, G., Esin, S., & Maisetta, G. (2023). Antibacterial and antibiofilm effects of lactobacilli strains against clinical isolates of Pseudomonas aeruginosa under conditions relevant to cystic fibrosis. Antibiotics, 12(7), 1158. https://doi.org/10.3390/antibiotics12071158
Belousov, A., Patlay, A., Silant'ev, V., Kovalev, V. V., & Kumeiko, V. (2022). Preparation of hydrogels based on modified pectins by tuning their properties for anti-glioma therapy. International Journal of Molecular Sciences, 24(1), 630. https://doi.org/10.3390/ijms24010630
Benassi, L., Alessandri, I., & Vassalini, I. (2021). Assessing green methods for pectin extraction from waste orange peels. Molecules, 26(6), 1766. https://doi.org/10.3390/molecules26061766
Carvalho, F. M., Mergulhão, F. J. M., & Gomes, L. C. (2021). Using lactobacilli to fight Escherichia coli and Staphylococcus aureus biofilms on urinary tract devices. Antibiotics, 10(12), 1525. https://doi.org/10.3390/antibiotics10121525
Chen, X., Bai, H., Mo, W., Zheng, X., Chen, H., Yin, Y., Liao, Y., Chen, Z., Shi, Q., Zuo, Z., Liang, Z., & Peng, H. (2025). Lactic acid bacteria bacteriocins: Safe and effective antimicrobial agents. International Journal of Molecular Sciences, 26(9), 4124. https://doi.org/10.3390/ijms26094124
Dafe, A., Etemadi, H., Dilmaghani, A., & Mahdavinia, G. R. (2017). Investigation of pectin/starch hydrogel as a carrier for oral delivery of probiotic bacteria. International Journal of Biological Macromolecules, 97, 536–543. https://doi.org/10.1016/j.ijbiomac.2017.01.060
Drumond, M. M., Tapia-Costa, A. P., Neumann, E., Nunes, Á. C., Barbosa, J. W., Kassuha, D. E., & Mancha-Agresti, P. (2023). Cell-free supernatant of probiotic bacteria exerted antibiofilm and antibacterial activities against Pseudomonas aeruginosa: A novel biotic therapy. Frontiers in Pharmacology, 14, 1152588. https://doi.org/10.3389/fphar.2023.1152588
Gao, T., Wang, X., Li, Y., & Ren, F. (2023). The role of probiotics in skin health and related gut–skin axis: A review. Nutrients, 15(14), 3123. https://doi.org/10.3390/nu15143123
Gonsalves, A., Tambe, P., Le, D., Thakore, D., Wadajkar, A. S., Yang, J., Nguyen, K. T., & Menon, U. J. (2021). Synthesis and characterization of a novel pH-responsive drug-releasing nanocomposite hydrogel for skin cancer therapy and wound healing. Journal of Materials Chemistry B, 9(46), 9533–9546. https://doi.org/10.1039/d1tb01934a.
Halder, D., Mandal, M., Chatterjee, S. S., Pal, N. K., & Mandal, S. (2017). Indigenous probiotic Lactobacillus isolates presenting antibiotic-like activity against human pathogenic bacteria. Biomedicines, 5(2), 31. https://doi.org/10.3390/biomedicines5020031
Hamed, R., Magamseh, K. H., Al-Shalabi, E., Hammad, A., Abu-Sini, M., Abulebdah, D. H., Tarawneh, O., & Sunoqrot, S. (2025). Green hydrogels prepared from pectin extracted from orange peels as a potential carrier for dermal delivery systems. ACS Omega, 10(17), 17182–17200. https://doi.org/10.1021/acsomega.4c08449
Han, S. S., Ji, S. M., Park, M. J., Suneetha, M., & Uthappa, U. T. (2022). Pectin-based hydrogels for drug delivery applications: A mini review.Gels, 8(12), 834. https://doi.org/10.3390/gels8120834
Hassan, M. U., Nayab, H., Rehman, T. U., Williamson, M. P., Haq, K. U., Shafi, N., & Shafique, F. (2020). Characterisation of bacteriocins produced by Lactobacillus spp. isolated from the traditional Pakistani yoghurt and their antimicrobial activity against common foodborne pathogens. BioMed research international, 2020(1), 8281623.
Hassan, M., Javadzadeh, Y., Lotfipour, F., & Badomchi, R. (2011). Determination of comparative minimum inhibitory concentration (MIC) of bacteriocins produced by enterococci for selected isolates of multi-antibiotic-resistant Enterococcus spp. Advanced pharmaceutical bulletin, 1(2), 75.
Hernández-González, J. C., Martínez-Tapia, A., Lazcano-Hernández, G., García-Pérez, B. E., & Castrejón-Jiménez, N. S. (2021). Bacteriocins from lactic acid bacteria. Animals, 11(4), 979. https://doi.org/10.3390/ani11040979
John, S. M., & Deeseenthum, S. (2015). Properties and benefits of kefir – A review. Songklanakarin Journal of Science and Technology, 37(3), 275–282
Karuppusamy, M., Kumar, S., Selvam, H., Sangapillai, K., Kamachisundaram, K., & Rama, B. (2024). Anti-quorum sensing activity and bioactive components of marine-derived bacteria. Journal of Pure and Applied Microbiology, 18(3), 9210–9220. https://doi.org/10.22207/JPAM.18.3.55
Khalfallah, G., Gartzen, R., Möller, M., et al. (2021). A new approach to harness probiotics against common bacterial skin pathogens: Towards living antimicrobials. Probiotics & Antimicrobial Proteins, 13, 1557–1571. https://doi.org/10.1007/s12602-021-09783-7
Li, Y., Yu, S., Weng, P., Wu, Z., & Liu, Y. (2023). Purification and antimicrobial mechanism of a novel bacteriocin produced by Lactiplantibacillus plantarum FB-2. LWT, 185, 115123. https://doi.org/10.1016/j.lwt.2023.115123
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J biol Chem, 193(1), 265-275
Citations |
 |
 |
 |
 |
