National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The international high-risk clone Pseudomonas aeruginosa ST773 has emerged as a clinically significant lineage associated with metallo-β-lactamases, particularly NDM-1, and extensive drug resistance. Cross-border spread and hospital outbreaks involving ST773 NDM-1 have been documented in Europe, South Africa, and Asia, emphasizing the need for routine genomic surveillance to define resistome content and infer mobile element contexts. Paired-end Illumina reads (metadata not provided) were quality-checked and trimmed (fastp), assembled de novo (SPAdes), and evaluated (QUAST). In silico MLST was assigned using the PubMLST P. aeruginosa scheme. AMR genes and resistance-associated point mutations were identified with AMRFinderPlus v4.2.7 using the P. aeruginosa organism model and database 2026-01-21.1. Plasmid reconstruction was attempted using MOB-suite v3.1.9 but could not be completed due to database initialization/download errors. Annotation was attempted using Prokka but was blocked by database setup/indexing failures. The draft genome assembly was ~7.09 Mb with N50 = 147,017 bp. MLST classified the isolate as ST773 (canonical allele profile acsA-5, aroE-4, guaA-5, mutL-5, nuoD-5, ppsA-7, trpE-8). The resistome included blaNDM-1 (NODE_146_length_1356_cov_49.874902; 100% identity), intrinsic/allelic β-lactamases (blaPDC-16, blaOXA-395), quinolone resistance determinants (gyrA_T83I, parC_S87L, qnrVC1), and integron/biocide-linked markers (sul1, qacEΔ1), among additional aminoglycoside and other resistance genes. The blaNDM-1-containing contig coverage (~50× as encoded in the SPAdes header) was broadly comparable to chromosomal-coverage contigs, supporting—but not proving—chromosomal/ICE localization consistent with ST773 NDM-1 outbreaks where blaNDM-1 resides in ~117 kb chromosomal ICEs. This clinical ST773 isolate carries blaNDM-1 and a multi-class resistome consistent with the expanding global footprint of ST773 NDM-1 lineages. The co-occurrence of integron-associated markers (qacEΔ1/sul1) with multiple resistance determinants underscores the potential for co-selection and persistence in hospital environments. Long-read sequencing and read-mapping are warranted to resolve blaNDM-1 genetic context and plasmid/ICE architecture.
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