National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study to optimize the growth parameters to significantly enhancing the production of antifungal metabolite by bioactive isolate Streptomyces pseudogriseolus VSG-9 isolated from soil sample collected on Gulbarga region Kalaburagi, India. The impact of different factors, including culture media, pH, temperature, agitation, carbon and nitrogen sources varying single parameters at a time. The antifungal activity of the crude extract was evaluated using agar well diffusion method. The Streptomyces pseudogriseolus VSG-9 exhibited the highest growth and metabolite production was achieved in Starch casein agar with supplement with % of dextrose at pH 7 and 350C temperature at stationary condition. A comparative study was performed to evaluate the antifungal activity of amphotericin B, fluconazole, ketoconazole, and nystatin along with extracellular and intracellular crude extract of S.pseudogriseolus against Candida strains. Ketoconazole and fluconazole showed antifungal activity against tested Candida sp., while amphotericin B, and nystatin exhibited resistance. Interestingly, both extract of S.pseudogriseolus VSG-9 demonstrated potent and broad spectrum of inhibitory effect against all tested Candida sp.
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