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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1804-1814
DOI: https://doi.org/10.20546/ijcmas.2017.604.216


Genetic Diversity among Pigeonpea (Cajanus cajan L. Millsp.) Genotypes Using Genic SSRs with Putative Function for Drought Tolerance
B. Sarkar, V.S.K. Chakravarthy, Y. Varalaxmi, S.K. Yadav, M. Vanaja and M. Maheswari*
Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad, Telangana State, India
*Corresponding author
Abstract:

One hundred and thirty eight pigeonpea genotypes were analyzed for molecular genetic diversity using 34 SSR markers with putative function for drought tolerance. The study revealed considerable molecular genetic diversity among genotypes. Fifty two alleles were obtained with 34 SSR markers while, 1 to 3 alleles was scored with an average of ~1.6 alleles for each SSR. Three alleles were amplified by markers ASSR1, ASSR93 and ASSR97. Of these, 15 SSR markers were found to be polymorphic which identified 33 alleles among 138 genotypes. The average PIC value of these polymorphic SSRs was 0.22 with a range of 0.01 for ASSR308 to 0.38 in ASSR97. Significant positive correlation was observed between PIC values with number of alleles amplified per primer (r = 0.58*, P < 0.05), and gene diversity (r = 0.99**, P < 0.01) and between allele number and gene diversity (r = 0.57*, P<0.05). The average genetic distance for all pair wise comparisons was estimated as 0.27. The highest genetic distance of 0.69 was recorded between genotypes PAU-881 and LRG-41. Cluster analysis, done by UPGMA following Nei's similarity matrix and population structure analyses grouped 138 pigeonpea genotypes into seven sub-populations.


Keywords: Molecular markers, SSR, Genetic diversity, Drought tolerance, Cluster analysis, Population structure
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How to cite this article:

Sarkar, B., V.S.K. Chakravarthy, Y. Varalaxmi, S.K. Yadav, M. Vanaja and Maheswari, M. 2017. Genetic Diversity among Pigeonpea (Cajanus cajan L. Millsp.) Genotypes Using Genic SSRs with Putative Function for Drought Tolerance. Int.J.Curr.Microbiol.App.Sci. 6(4): 1804-1814. doi: https://doi.org/10.20546/ijcmas.2017.604.216