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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2695-2711
DOI: https://doi.org/10.20546/ijcmas.2017.604.314


Desulfurization of Crude Oil and Oil Products by Local Isolated Bacterial Strains
Ahmad F. Shahaby1,2* and Khaled. M. Essam El-din1
1Scientific Research Deanship, Biotechnology and Genetic Engineering Unit,
Taif University, Taif, Saudi Arabia
2 Department of Microbiology, College of Agriculture, Cairo University, Cairo, Egypt
*Corresponding author
Abstract:

The presence of organic sulfur-containing oil in the environment is harmful to animals and human health. The combustion of these compounds in fossil fuels tends to release sulfur dioxide in the atmosphere, which leads to acid rain, corrosion, damage to crops, and an array of other problems. The process of biodesulfurization rationally exploits the ability of certain microorganisms in the removal of sulfur prior to fuel burning, without loss of calorific value. In this sense, we hypothesized that bacterial isolates from crude oil and oil products polluted soils can demonstrate the ability to degrade crude oil and oil products as well as dibenzothiophene (DBT), the major sulfur-containing compound present in fuels. The total sulfur bacteria were ranged from 1.6x104- 2.8x106 CFU g soil-1 on PCA media and 4.1x102- 2.1x106 CFU g soil-1 on basel media (BSM) supplemented with DBT. Those strains which showed great degradation efficiency in case of all investigated hydrocarbons were identified based upon the sequence analysis of their 16S- rRNA. Phenotypic and genotypic examination of the recovered isolates revealed that they belong to the five different genera of Bacillus, Pseudomonas, Rodococcus, Mycobacterium, and Klebsiella. All isolated bacteria showed to be capable of biodesulfurization of oil or oil products, as they were compared to standard strains (ATCC) and were able to grow in minimal mineral medium supplemented with DBT or 2HBP as the sole sulfur and carbon source. The potential application of these isolates for the biodesulfurization of oil and oil products as well as sulfur-containing compound in fuels prior to combustion was discussed. Furthermore, results indicated that using a microbial consortium might have a promising application in petroleum oil technology and could be potentially used in microbial enhanced oil recovery (MEOR).


Keywords: Crude oil, Oil products, Dibenzothiophene, Biodesulfurization, Bacillus, Pseudomonas, Klebsiella, Mycobacterium, Rhodococcus,16S-rRNA gene
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How to cite this article:

Ahmad F. Shahaby and Khaled. M. Essam El-din. 2017. Desulfurization of Crude Oil and Oil Products by Local Isolated Bacterial StrainsInt.J.Curr.Microbiol.App.Sci. 6(4): 2695-2711. doi: https://doi.org/10.20546/ijcmas.2017.604.314