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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1318-1325
DOI: https://doi.org/10.20546/ijcmas.2017.604.161


Production and Optimization of Endoglucanase by Aspergillus sp., Trichoderma sp. and Penicillium sp
Kirit B. Patel1*, S.S. Patel2, B.K. Patel1, H.C. Chauhan2, Manish Rajgor2, J.K. Kala2, M.A. Patel2, M.G. Patel2, A.C. Patel2, M.D. Shrimali2, A.N. Modi2 and B.S. Chandel2
1Ashok and Rita Patel Institute of Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388121, Sardar Patel University V.V.Nagar-388120, Gujarat, India
2Department of Animal Biotechnology and Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar-385506, Gujarat, India
*Corresponding author
Abstract:

Endoglucanase is one of the most important industrial enzymes. In the present study, three different fungi Aspergillus sp., Trichoderma sp. and Penicillium sp. were isolation for endoglucanase production from soil samples on PDA plate. All three fungus processed by submerged fermentation and solid state fermentation for its enzymatic activity. Aspergillus sp., showed highest enzymatic activity in both type of fermentation. In solid state fermentation using sawdust, corncob and wheat bran substrate, all three fungus give highest enzyme activity in corncob substrate as compared to sawdust and wheat bran substrate. The optimization of endoglucanase activity of Aspergillus sp., Trichoderma sp. and Penicillium sp. with different parameter like pH, temperature, carbon source, nitrogen source, CMC concentration and incubation period. Aspergillus sp. give maximum endo-ß-glucanase activity at pH 3, 28oC temperature, corn starch as a carbon source, sodium nitrate as a nitrogen source, 1.5% CMC concentration and 6 days incubation period.


Keywords: Submerged fermentation, Endo-ß-glucanase, Optimization
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How to cite this article:

Kirit B. Patel, S.S. Patel, B.K. Patel, H.C. Chauhan, Manish Rajgor, J.K. Kala, M.A. Patel, M.G. Patel, A.C. Patel, M.D. Shrimali, A.N. Modi and Chandel, B.S. 2017. Production and Optimization of Endoglucanase by Aspergillus sp., Trichoderma sp. and Penicillium spInt.J.Curr.Microbiol.App.Sci. 6(4): 1318-1325. doi: https://doi.org/10.20546/ijcmas.2017.604.161