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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:4, April, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 783-788
DOI: https://doi.org/10.20546/ijcmas.2017.604.098


Isolation and Screening of Amylase Producing Fungi
Sujeeta, Kamla Malik*, Shikha Mehta and Khushboo Sihag
Department of Microbiology, College of Basic Sciences and Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana-125004, India
*Corresponding author
Abstract:

The purpose of this study was to isolate and screened amylase producing fungi from different sources. A total of twenty amylase producing fungal isolates were obtained from soil, fruits, vegetables, baked food, and flour on the starch mineral agar medium. It was observed that ten fungi and ten yeasts isolates produced amylase, only nine fungal colonies showed positive results for amylase production. The maximum amylase activity shown by yeast isolates YPO3 (3.2cm) followed by YBR2 (3.1cm). Preliminary morphological observations of selected fungal isolates colonies were black, green, white, creamy, yellow, reddish, powdery, flat, round, raised and shiny. The highest enzyme activity was produced by YPO3 (13.72 U/ml) isolate after 120 hr of incubation.


Keywords: Amylase, starch mineral agar medium, yeast isolates, Fungi

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How to cite this article:

Sujeeta, Kamla Malik, Shikha Mehta and Khushboo Sihag. 2017. Isolation and Screening of Amylase Producing FungiInt.J.Curr.Microbiol.App.Sci. 6(4): 783-788. doi: https://doi.org/10.20546/ijcmas.2017.604.098
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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