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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1-13
DOI: https://doi.org/10.20546/ijcmas.2017.604.001


Antimicrobial Activities and Cytotoxicity of Sisymbrium irio L Extract against Multi-Drug Resistant Bacteria (MDRB) and Candida albicans
Gamal M. El-Sherbiny, Saad A.M. Moghannem and Mohammed H. Sharaf
 1Department of Botany and Microbiology, Faculty of Science, Al
Azhar University, Egypt
2Department of Botany and Microbiology, Faculty of Science, Al
Azhar University, Holding Company for Biological Product and Vaccine (VACSERA), Agouza, Giza, Egypt
*Corresponding author
Abstract:

The present study was conducted to evaluate antimicrobial and cytotoxicactivity of Sisymbrium irio L extract against MDRB (Staphylococcus aureus, Enterococcus faecium, Klebsiella pneumoniae, Acinetobacter baumanni, Enterobacter cloacae and Pseudomonas aeruginosa) and Candida albicans. Antibiotic sensitivity profile was performed using disc diffusion method. Cytotoxicity was measured against African Green Monkey Kidney (VERO) cell line using the colorimetric MTT assay. Antimicrobial activity of aqueous Sisymbrium irio L extract showed inhibition activity against tested organisms Staphylococcus aureus (17mm), Enterococcus faecium (22mm), Klebsiella pneumoniae (15mm), Acinetobacter baumanni (18mm), Enterobacter cloacae (17mm), Pseudomonas aeruginosa (15mm), and Candida albicans (21mm). The highest activity was observed against Enterococcus faecium. This inhibition activity was higher than most of antibiotics used in the study. The crude extract was purified using column chromatography and visualized under UV using Thin Layer Chromatography (TLC). The minimum inhibitory concentration (MIC) values of purified active compound ranged from 31.25 to 125μg/ml while the minimum bactericidal concentration (MBC)was two-fold higher than MIC ranged from 62.5 to 250μg/ml. Cytotoxic activity of purified active compound showed little toxicity withIC50 exceeding400μg/ml after 24hr of incubation. The purified compound was identified using UV, IR, 1HNMR and mass spectroscopy. The analysis of data obtained indicated that it belongs to cyclo hexanone group.


Keywords: Antibacterial activity, MDR, Cytotoxicity, Pathogenic fungi, Clinical isolates, Medicinal plants, Plant extract.
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How to cite this article:

Gamal M. El-sherbiny, Saad A.M. Moghannem and Mohammed H. Sharaf. 2017. Antimicrobialactivitiesand cytotoxicity of Sisymbrium irio L extract against Multi-Drug Resistant Bacteria (MDRB) and Candida albicans.Int.J.Curr.Microbiol.App.Sci. 6(4): 1-13. doi: https://doi.org/10.20546/ijcmas.2017.604.001