National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Conventional propagation of Anthurium (Anthurium andraeanum [Lind.]) plant through seeds and suckers is a tedious and very slow process and not practical when carried out commercially on large scale. Hence, biotechnological approach such as micropropagation is considered as a novel method for production of pathogen-free true-to-type plantlets at a faster rate. In the present study, a proficient surface sterilization procedure was standardized for aseptic culture from leaf explants of Anthurium cv. Xavia, under in-vitro conditions. The leaf explants were exposed to 0.1% HgCl2 for 2, 3, 4, 5, 6, 7 and 8 min) and control (distilled water) were cultured on MS media. The leaf explants exposed for 5 minutes resulted in minimum fungal (0.00%) and bacterial (0.18%) contamination with maximum survival percentage (97.66%) at 60 days after inoculation. The most effective surface sterilization time was taken into consideration for further sterilization explants and in-vitro culture in the MS basal media supplemented with of bio-regulators along with control for production of pathogen-free callus development of anthurium.
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