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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 2850-2856
DOI: https://doi.org/10.20546/ijcmas.2018.709.354


Impact of Sterilization Exposure Time on Leaf Explants of Anthurium cv. Xavia for Establishing an Aseptic Culture
Rashmita Toppo1 and Sashikala Beura2
1Floriculture and Landscaping, College of Agriculture, OUAT, India
2Floriculture and Landscaping, College of Agriculture and BTCC, OUAT, India
*Corresponding author
Abstract:

Conventional propagation of Anthurium (Anthurium andraeanum [Lind.]) plant through seeds and suckers is a tedious and very slow process and not practical when carried out commercially on large scale. Hence, biotechnological approach such as micropropagation is considered as a novel method for production of pathogen-free true-to-type plantlets at a faster rate. In the present study, a proficient surface sterilization procedure was standardized for aseptic culture from leaf explants of Anthurium cv. Xavia, under in-vitro conditions. The leaf explants were exposed to 0.1% HgCl2 for 2, 3, 4, 5, 6, 7 and 8 min) and control (distilled water) were cultured on MS media. The leaf explants exposed for 5 minutes resulted in minimum fungal (0.00%) and bacterial (0.18%) contamination with maximum survival percentage (97.66%) at 60 days after inoculation. The most effective surface sterilization time was taken into consideration for further sterilization explants and in-vitro culture in the MS basal media supplemented with of bio-regulators along with control for production of pathogen-free callus development of anthurium.


Keywords: Anthurium, Sterilization, Survival, HgCl2
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How to cite this article:

Rashmita Toppo and Sashikala Beura. 2018. Impact of Sterilization Exposure Time on Leaf Explants of Anthurium cv. Xavia for Establishing an Aseptic Culture.Int.J.Curr.Microbiol.App.Sci. 7(9): 2850-2856. doi: https://doi.org/10.20546/ijcmas.2018.709.354