Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 478-493
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.054


Plasmid Profile of Multiple Antibiotics Resistant (MAR) Bacteria Isolated from Leachate Samples in Ebira Communities of Ekiti Central and Ekiti South, Ekiti State, Nigeria
A.T. Odeyemi*, O.S. Fasuan and O.Y. Olufowora
Microbiology Department, Ekiti State University, Ado-Ekiti, Nigeria
*Corresponding author
Abstract:

Plasmid curing of bacteria isolated from leachate samples in Ebira communities of Ekiti Central and South in Ekiti State senatorial district were analyzed. Microbial count, antibiotics sensitivity and plasmid profile were assayed using pour plate, disk diffusion and gel electrophoresis techniques respectively; while the plasmids were cured using acridine orange. The total bacterial, total coliform and total enteric bacteria counts of the leachate samples ranged from 0.64 × 108 to 0.85 × 109CFU/ml, 0.78 × 108 to 0.76 × 109CFU/ml and 1.90 × 108 to 2.30 × 109CFU/ml respectively. The percentage distributions revealed that Escherichia coli with 29.2% had the highest frequency of occurrence while Enterobacter aerogenes had the lowest occurrence of 7.5%. The antibiotics sensitivity test showed that the least percentage (3%) of the isolates were resistant to ofloxacin while the highest percentage (99%) of the isolates were resistant to cefuroxime. Multiple Antibiotics Resistant bacteria were subjected to plasmid analysis showing that nine (9) were devoid of plasmid while other six (6) isolates carry plasmid with high molecular weight, ranging from 9.41kbp to 23.130kpb. Three of the MAR isolates (Escherichia coli, Pseudomonas aeruginosa and Enterococcus feacalis) were selected for curing. The three (3) retained resistance pattern to all antibiotics used aside Escherichia coli and Pseudomonas aeruginosa that became susceptible to ofloxacin after curing. Incidence of such MAR bacterial isolates in leachate samples is however an indication of possible environmental hazard.


Keywords: Plasmid curing, leachate,MAR,Ebira Communities.
Download this article as Download

How to cite this article:

Odeyemi, A.T., O.S. Fasuan and O.Y. Olufowora. 2016. Plasmid Profile of Multiple Antibiotics Resistant (MAR) Bacteria Isolated from Leachate Samples in Ebira Communities of Ekiti Central and Ekiti South, Ekiti State, Nigeria.Int.J.Curr.Microbiol.App.Sci. 5(10): 478-493. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.054