International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 459-466

Characterization of Alternaria alternata Isolated from Calotropis gigantea Plant Leaf
Prabhat Kumar Mishra* and Vijay Thawani
Centre for Scientific Research and Development,People’s University, Bhanpur Bhopal - 462037 (Madhya Pradesh) India
*Corresponding author

Calotropis (madar) is used as a traditional medicine either alone or with other actives in the treatment of fever, rheumatism, indigestion, cough, cold, eczema, asthma, elephantiasis, nausea, vomiting, and diarrhea. In Ayurveda the dried whole plant is recommended as a tonic, expectorant, depurative, harbs and anitparasitic. The dried root bark is a substitute for vomiting. The flowers are bitter, digestive, astringent, stomachic, anthelmintic, vermicide and tonic. Alternaria alternata isolated from Calotropis gigantea showed excellent growth in potato carrot agar (PCA) medium with a colony diameter of 2 cm after 4 days of incubation at 27ËšC for 8 days and declined from 11th day of incubation. Alternaria alternata favoured slightly acidic and neutral ph range while poor mycelia development was observed in alkaline medium. At alkaline ph, hydrogen ion concentration had inhibitory effect on the growth and development of mycelia and pigmentation of the fungus. Alternaria alternata could grow at a wide range of temperature between 20-30ËšC. Best growth and sporulation of the fungus was observed at 27ËšC, with colony diameter of 2.6 cms and sporulation. At 27ËšC, the colony diameter was 2.6 cm and sporulation was good. The fungus attained its best growth and sporulation at 95 and 100% RH, with colony diameter measuring 2.6 and 2.9cm respectively. Alternaria alternata appears from June to August at temperature of 25 to 30ºC.  

Keywords: pH,temperature, media,cultural characteristics.

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How to cite this article:

Prabhat Kumar Mishra and Vijay Thawani. 2016. Characterization of Alternaria alternata Isolated from Calotropis gigantea Plant Leaf.Int.J.Curr.Microbiol.App.Sci. 5(10): 459-466. doi:
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