National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study, silver nanoparticles (AgNPs) using leaf extracts of Azadirachta indica were synthesized and its antibacterial activity was investigated. The synthesis of AgNPs was done by using the green synthetic method and analyzed by UV-Visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The antibacterial activity of AI AgNPs was studied by agar well diffusion method. The prepared AI AgNPs showed characteristic absorption peak at 440 nm in the UV-Vis spectrum. Fourier transform infrared spectra had shown that the biomolecules present in leaf extract were responsible for the reduction and capping material of silver nanoparticles. Transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 20-50 nm. The AI AgNPs showed good antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Staphylococcus aureus. In agar well diffusion method, the maximum zone of inhibition was found against Escherichia coli with 30 mm and minimum zone of inhibition was found to be against Bacillus cereus with 21 mm. The present study explored that Azadirachta indica which are efficient producers of AgNPs and could act as safe and cost-effective with potential antibacterial activities. These findings encourage studying AI AgNP further for their potential biological applications.
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