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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3636-3642
DOI: https://doi.org/10.20546/ijcmas.2018.701.427


Green Synthesis of Nanosilver from Azadirachta indica Leaves and Its Antibacterial Activity
S. Bharathi1, D. Saravanan2* and M. S. Nalina Sundari1
1Department of Zoology, Queen Mary’s College, Chennai – 600 004, Tamil Nadu, India 2Centre for Laboratory Animal Technology and Research, Sathyabama University, Chennai - 600 119, Tamil Nadu, India
*Corresponding author
Abstract:

In this study, silver nanoparticles (AgNPs) using leaf extracts of Azadirachta indica were synthesized and its antibacterial activity was investigated. The synthesis of AgNPs was done by using the green synthetic method and analyzed by UV-Visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The antibacterial activity of AI AgNPs was studied by agar well diffusion method. The prepared AI AgNPs showed characteristic absorption peak at 440 nm in the UV-Vis spectrum. Fourier transform infrared spectra had shown that the biomolecules present in leaf extract were responsible for the reduction and capping material of silver nanoparticles. Transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 20-50 nm. The AI AgNPs showed good antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Staphylococcus aureus. In agar well diffusion method, the maximum zone of inhibition was found against Escherichia coli with 30 mm and minimum zone of inhibition was found to be against Bacillus cereus with 21 mm. The present study explored that Azadirachta indica which are efficient producers of AgNPs and could act as safe and cost-effective with potential antibacterial activities. These findings encourage studying AI AgNP further for their potential biological applications.


Keywords: Green synthesis, Azadirachta indica, silver nanoparticles, antibacterial
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How to cite this article:

Bharathi S., D. Saravanan and Nalina Sundari M. S. 2018. Green Synthesis of Nanosilver from Azadirachta indica Leaves and Its Antibacterial Activity.Int.J.Curr.Microbiol.App.Sci. 7(1): 3636-3642. doi: https://doi.org/10.20546/ijcmas.2018.701.427