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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 386-396
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.044


Bioremediation of Poly Aromatic Hydrocarbons (PAHs) and Crude Oil by Fungal Consortium from West Coast of Karnataka, India
S.V. Praveen Kumar, B.K. Manjunatha*, Pavani Bhat, R. Veena, Swetha S. Pawate and M. Yogashree
Department of Biotechnology, The Oxford College of Engineering,Bengaluru-560068 Karnataka, India
*Corresponding author
Abstract:

The present study primarily aims at developing an effective fungal consortium for the bioremediation of Poly Aromatic Hydrocarbons (PAHs) and crude oil. The hydrocarbon utilization capabilities of the potent isolated strains were studied for test hydrocarbons like benzene, toluene, naphthalene, paraffin and crude oil as model hydrocarbons for a period of 10 days. Degradation process was assessed by measuring percentage of TPH in residual oil, biological oxygen demand (BOD), chemical oxygen demand (COD), total plate count, UV spectrophotometric analysis and GC-MS studies. The study revealed that, among 45 isolated strains from PAHs/crude oil contaminated soil; four strains showed maximum degradation of crude oil & PAHs.18s rRNA sequencing was used to identify the strains and, was identified as Aspergillus terreus, Aspergillus aculeatus, Scedosporium boydii and Aspergillus sp. The degradation process for this consortium was further optimized using Response Surface Method (RSM). The fungal isolates were also screened for biosurfactant production, Immobilization of the isolates on sodium alginate beads will be used as model of bioremediation studies.


Keywords: Bioremediation, Fungi,West Coast,PAHs,Crude oil, GC-MS.
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How to cite this article:

Praveen Kumar, S.V., B.K. Manjunatha, Pavani Bhat, R. Veena, Swetha S. Pawate and Yogashree, M. 2016. Bioremediation of Poly Aromatic Hydrocarbons (PAHs) and Crude Oil by Fungal Consortium from West Coast of Karnataka.Int.J.Curr.Microbiol.App.Sci. 5(10): 386-396. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.044