National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study primarily aims at developing an effective fungal consortium for the bioremediation of Poly Aromatic Hydrocarbons (PAHs) and crude oil. The hydrocarbon utilization capabilities of the potent isolated strains were studied for test hydrocarbons like benzene, toluene, naphthalene, paraffin and crude oil as model hydrocarbons for a period of 10 days. Degradation process was assessed by measuring percentage of TPH in residual oil, biological oxygen demand (BOD), chemical oxygen demand (COD), total plate count, UV spectrophotometric analysis and GC-MS studies. The study revealed that, among 45 isolated strains from PAHs/crude oil contaminated soil; four strains showed maximum degradation of crude oil & PAHs.18s rRNA sequencing was used to identify the strains and, was identified as Aspergillus terreus, Aspergillus aculeatus, Scedosporium boydii and Aspergillus sp. The degradation process for this consortium was further optimized using Response Surface Method (RSM). The fungal isolates were also screened for biosurfactant production, Immobilization of the isolates on sodium alginate beads will be used as model of bioremediation studies.
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