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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 379-385
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.043


Potential of Halophilic Bacteria for Extracellular Enzymes
K.D. Kamble*
Department of Microbiology, Sant Gadge Baba Amravati University,Amravati, Maharashtra, India
*Corresponding author
Abstract:

Although protease, amylase and lipase are frequently studied; in the list L-asparaginase also finds position. Halophilic enzymes are currently preferred because these remains active at wide range of pH and temperature and also the yield of purified functional enzymes is high. Having studied around 29 bacteria for DNA degrading abilities; as extended part of the study these bacteria are undertaken to determine their potential for protease, amylase, lipase and L-asparaginase productions. The study included members of genera Providencia, Rheinheimera, Halomonas, Alishewanella, Serratia, Marinobacter, Marinospirillum, Bacillus, Exiguobacterium and Vibrio species. The enzymatic activities were observed in terms of detectable zones of hydrolysis which were measured. The zones of hydrolysis appeared after 24 h and were at its maximum towards 48 h. The results revealed enzyme activities largely varied among genus and within genus also. Amylase was efficiently produced by Bacillus cereus (DN3), Vibrio species (DN25), Marinobacter species (DN16) whereas excellent protease activity was exhibited by Bacillus cereus (DN3), Bacillus thuringiensis (DN2) and Vibrio Sp (DN25). Exiguobacterium (SK1 and SK2) was found to be the most efficient lipase producing bacteria followed by Providencia rettgeri (DN1) and Bacillus thuringiensis (DN2). The therapeutic enzyme L-asparaginase was produced only by two bacteria i.e. Pseudomonas (DN13) and Halomonas (DN19). Looking at industrial market of the enzymes; it is suggested that these bacteria would be promising sources of stable and robust enzymes.


Keywords: Halophiles, bacteria,enzymes, efficiency L-asparaginase
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How to cite this article:

Kamble, K.D. 2016. Potential of Halophilic Bacteria for Extracellular Enzymes.Int.J.Curr.Microbiol.App.Sci. 5(10): 379-385. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.043