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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1318-1323
DOI: https://doi.org/10.20546/ijcmas.2018.709.157


Molecular Diagnosis of a Cutaneous Form of Pox in Pigeons at Mhow in Madhya Pradesh
S.D. Audarya1*, T. Riyesh2, N. Kumar2, D. Chhabra1, R. Sikrodia1, R. Sharda1, S. Barua2 and U.K. Garg3
1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453446, Madhya Pradesh, India
2National Center for Veterinary Type Cultures, Indian Council of Agricultural Research-National Research Center on Equines, Sirsa Road, Hisar-125001, Haryana, India
3Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453446, Madhya Pradesh, India
*Corresponding author
Abstract:

Avian poxviruses cause pox in different kind of birds. In the present study, investigations of a cutaneous form of pox in pigeons were reported. In June 2017, a Pigeon showing clinical lesions of yellowish crust/nodules on and around beak and eyes and feet was observed in a scattered group of around 40 pigeons. Lesions were collected aseptically in a container with 50% glycerol phosphate buffer solution. After processing of clinical specimen the inoculums were inoculated into chicken embryonated eggs by using chorioallantoic membrane route. Viral deoxyribonucleic acid was extracted and used in Avipox virus specific polymerase chain reaction. No specific lesions were observed in inoculated chicken embryonated egg even after third passage. However, in polymerase chain reaction Avipox virus specific P4b gene (578 bp) was successfully amplified and visualized in 1% agarose gel on electrophoresis. Clinical signs and amplification of Avipox virus specific gene confirmed cutaneous form of Pox in Pigeons at Mhow in Madhya Pradesh.


Keywords: Avian pox, P4b gene, PCR, Pigeon
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How to cite this article:

Audarya, S.D., T. Riyesh, N. Kumar, D. Chhabra, R. Sikrodia, R. Sharda, S. Barua and Garg, U.K. 2018. Molecular Diagnosis of a Cutaneous Form of Pox in Pigeons at Mhow in Madhya Pradesh.Int.J.Curr.Microbiol.App.Sci. 7(9): 1318-1323. doi: https://doi.org/10.20546/ijcmas.2018.709.157