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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 894-900
DOI: https://doi.org/10.20546/ijcmas.2018.709.107


Molecular Marker Assisted Confirmation of Hybridity in Indian mustard (Brassica juncea L.)
Manmohan Sharma, Dechen Dolkar, R.K. Salgotra, Deepika Sharma, Punya, Amrinder Singh and S.K. Gupta
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (J&K), India
*Corresponding author
Abstract:

The present study was undertaken to confirm the hybridity of Brassica juncea L. F1 plants based on the amplification pattern of SSR markers. The complementary banding pattern of the male and female parents helped to confirm the genuineness of F1 plants developed. The seeds of two genotypes of Brassica juncea, RSPR-01 used as female parent and Donskaja-IV used as male parent were raised in pots under field conditions and crossed to develop F1 seeds. Out of 20 random SSR primers used for the screening of parental genotypes for polymorphism, 5 primers were found polymorphic. Based on the complementary banding patterns between the hybrid plants and parents, the polymorphic SSR markers BR_A04_9627743 and BR_A01_13393871 were identified as the specific markers which enable to distinguish and identify hybrid form their parental lines. The expression of both the parental alleles in six out of the seven plants confirmed their true hybrid nature.


Keywords: Brassica juncea L., Hybridity, SSR markers, Polymorphism, Alleles
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How to cite this article:

Manmohan Sharma, Dechen Dolkar, R.K. Salgotra, Deepika Sharma, Punya, Amrinder Singh and Gupta, S.K. 2018. Molecular Marker Assisted Confirmation of Hybridity in Indian mustard (Brassica juncea L.).Int.J.Curr.Microbiol.App.Sci. 7(9): 894-900. doi: https://doi.org/10.20546/ijcmas.2018.709.107