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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 289-293
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.032


Speciation and Antifungal Susceptibility of Candida Species Isolated from Clinical Samples – A Pilot Study
M. Saleem1*, R. Gopal1, T. Mangaiyarkarsi1, S. Sunil1, J. Krishnapriya1 and R. Nagma2
1Department of Microbiology, Sri Manakula Vinayagar Medical College and Hospital, Puducherry, India
2Department of Microbiology, SMVMCH, Puducherry, India
*Corresponding author
Abstract:

Infections due to Candida species are the most common fungal infections. The increase in the population at risk of developing invasive candidiasis viz. immunocompromised patients, patients with diabetes mellitus, prolonged use of antibiotics, immunosuppressive therapy has resulted in a rise in infections due to non-albicans candida species which are resistant to fluconazole. All candida species isolated from various clinical specimens during a period of 6 months (Jan 2016 to June 2016) were speciated by conventional tests and grown on SDA, chlamydospore agar and chrome agar. All Candida albicans isolates were tested for susceptibility to ‘Fluconazole’ by disc diffusion. A total of 54 specimens yielded Candida species. Non-albicans Candida species constituted the predominant isolates and the highest yield was from urine samples. Candida albicans was isolated from 20 of the 54 specimens. Only 65% of the Candida albicans were susceptible to fluconazole. Chrome agar was found to be very useful in isolation and speciation of the Candida species. Non-albicans Candida is the most common cause of candidiasis in our region. Resistance to fluconazole among Candida albicans is high (35%).


Keywords: Candida, speciation, fluconazole, resistance,non-albicans candida species
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How to cite this article:

Saleem, M., R. Gopal, T. Mangaiyarkarsi, S. Sunil, J. Krishnapriya and Nagma, R. 2016. Speciation and Antifungal Susceptibility of Candida Species Isolated from Clinical Samples – A Pilot Study.Int.J.Curr.Microbiol.App.Sci. 5(10): 289-293. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.032