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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 401-405
DOI: https://doi.org/10.20546/ijcmas.2018.709.049


Isolation, Identification and Molecular Detection of Brucella spp., in Cattle and Buffaloes
Sai Lakshmi Kanth Katrapati, N.S. Sharma* and Paviter Kaur
Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Corresponding author
Abstract:

The present study was carried out for the isolation, identification and molecular characterization of Brucella species. A total of 50 samples were collected from cattle and buffalo suffering from abortions and other reproductive disorders in and around Ludhiana, Punjab. Out of the 50 samples of fetal stomach contents (25), uterine discharges (10), vaginal mucus (8) and placenta (7) processed for isolation of Brucella of which a total of four isolate were obtained and identified biochemically. All the 4 isolates were typed as biotype 1. DNA was extracted from the solates and subjected to PCR using B4/B5 primer pair. All the isolates were positive by PCR and an amplicon size of 223bp was obtained.


Keywords: Isolation, Molecular Detection, Brucella spp., Buffaloes
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How to cite this article:

Sai Lakshmi Kanth Katrapati, N.S. Sharma and Paviter Kaur. 2018. Isolation, Identification and Molecular Detection of Brucella spp., in Cattle and Buffaloes.Int.J.Curr.Microbiol.App.Sci. 7(9): 401-405. doi: https://doi.org/10.20546/ijcmas.2018.709.049