International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 254-260

Detection of Aspergillus Species by Polymerase Chain Reaction
Raksha Singh1, Gurjeet Singh2* and A.D. Urhekar1
1Department of Microbiology, MGM Medical College and Hospital, MGM Institute of Health Sciences, Kamothe, Navi Mumbai-410209, Maharashtra, India
2Department of Microbiology, NC Medical College and Hospital, Israna,Panipat-132107, Haryana, India
*Corresponding author

Aspergillus species have recently caused increasing numbers of acute invasive infections in immunocompromised patients. This prospective and experimental study was carried out at Department of Microbiology and Central Research Laboratory, MGM Medical College and Hospital, Navi Mumbai. Detection of Aspergillus species was done using Polymerase Chain Reaction. This detection level (sensitivity 25%) is higher than Culture (19.5%), Fluorescence (17%) and Microscopy (10%) and difference in sensitivity is statically significant P value (0.001) <0.05, Significant. Species detection in nested PCR was A. niger (50%), A.fumigatus (35%) and A.flavus (15%). This shows more species specificity of PCR. Molecular methods are more sensitive and specific methods. It gives results for detection and speciation in short time which helps early diagnosis and treatment of patient.


Aspergillus, Polymerase Chain Reaction, Sensitivity,Patient

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How to cite this article:

Raksha Singh, Gurjeet Singh and A.D. Urhekar. 2016. Detection of Aspergillus Species by Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 5(10): 254-260. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.