Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 254-260
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.028


Detection of Aspergillus Species by Polymerase Chain Reaction
Raksha Singh1, Gurjeet Singh2* and A.D. Urhekar1
1Department of Microbiology, MGM Medical College and Hospital, MGM Institute of Health Sciences, Kamothe, Navi Mumbai-410209, Maharashtra, India
2Department of Microbiology, NC Medical College and Hospital, Israna,Panipat-132107, Haryana, India
*Corresponding author
Abstract:

Aspergillus species have recently caused increasing numbers of acute invasive infections in immunocompromised patients. This prospective and experimental study was carried out at Department of Microbiology and Central Research Laboratory, MGM Medical College and Hospital, Navi Mumbai. Detection of Aspergillus species was done using Polymerase Chain Reaction. This detection level (sensitivity 25%) is higher than Culture (19.5%), Fluorescence (17%) and Microscopy (10%) and difference in sensitivity is statically significant P value (0.001) <0.05, Significant. Species detection in nested PCR was A. niger (50%), A.fumigatus (35%) and A.flavus (15%). This shows more species specificity of PCR. Molecular methods are more sensitive and specific methods. It gives results for detection and speciation in short time which helps early diagnosis and treatment of patient.


Keywords:

Aspergillus, Polymerase Chain Reaction, Sensitivity,Patient

Download this article as Download

How to cite this article:

Raksha Singh, Gurjeet Singh and A.D. Urhekar. 2016. Detection of Aspergillus Species by Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 5(10): 254-260. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.028