National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Oxidative stress is caused by an imbalance between the production of reactive oxygen species (ROS) and the biological system capacity to either rapidly detoxify the precursor reagents, or to repair the resulting damage. All ROS are associated to several illnesses and health disorders like cancer, aterosclerosis, Alzheimer´s disease, diabetes and age related deterioration amongst others. The reason for that relies in the fact that ROS react with biomolecules such as lipids, proteins and DNA, and neutralise their function. Previous reports have proven that the phycobiliprotein extract of some species of algae and cyanobacteria acts as a free radical scavenger. The aim of this project was to observe the antioxidant effects of a raw phycobiliprotein extract of Arthrospira maxima in the survival of Saccharomyces cerevisiae previously submitted to oxidative stress through the formation of –OH radicals as result of the Fenton reaction, using ascorbic acid and Cu(II). S. cerevisiae strain was grown in agar Sabouraud, and then transferred to an oxidative stress treatments in presence and absence of raw phycobiliprotein extract of Arthrospira maxima. The results show that the survival of S. cerevisiae in dilutions of 10-2 and 10-3, go from 7 X 105 to zero in the presence or absence of the mentioned raw extract. Therefore, applying a non-parametric statistical test, a meaningful difference between treatments was obtained. All this led us to conclude that the presence of phycobiliproteins and their tetrapirrolic structure, reaction with ROS and enhances the growth of Saccharomyces cerevisiae in an oxidative media.
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