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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 4381-4391
DOI: https://doi.org/10.20546/ijcmas.2018.707.511


Bioremediation and Detoxification of Trypan Blue by Bacillus sp. Isolated from Textile Effluents
P. Jeevitha, D. Manjula, I. Ramya and J. Hemapriya*
Department of Microbiology, DKM College for women, Vellore, India
*Corresponding author
Abstract:

Azo dyes are commonly used in many commercial industries. 16 bacterial isolates were isolated from textile effluents, of which 4 isolates (HB1, HB2, HB3 and HB4) showed ability to decolorize Trypan blue dye. Based on the standard morphological and biochemical characteristics, HB3 isolate that showed maximum decolorization of Trypan blue was identified as Bacillus sp. HB3 isolate showed 96.6 % decolorization of Trypan blue within 24 h of incubation. Maximum decolorization of Trypan blue was found to be achieved at 35 °C, neutral pH in the presence of glucose (Carbon source) and Yeast extract (Nitrogen source). The activity of azo reductase, lignin peroxidase, tyrosinase, manganese Peroxidase was investigated for their role in biodegradation of Trypan blue. Specific activity of the azoreductase enzyme was found to be 0.46 U mg -1 protein. The crude protein extract subjected to SDS-PAGE resulted in the formation of a clear band (original band) against blue back ground which indicated the location of active azoreductase enzyme


Keywords: Azo dyes, Trypan blue, Bacillus sp., Azoreductase, Lignin Peroxidase
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How to cite this article:

Jeevitha P., D. Manjula, I. Ramya and Hemapriya J. 2018. Bioremediation and Detoxification of Trypan Blue by Bacillus sp. Isolated from Textile Effluents.Int.J.Curr.Microbiol.App.Sci. 7(7): 4381-4391. doi: https://doi.org/10.20546/ijcmas.2018.707.511