National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Azo dyes are commonly used in many commercial industries. 16 bacterial isolates were isolated from textile effluents, of which 4 isolates (HB1, HB2, HB3 and HB4) showed ability to decolorize Trypan blue dye. Based on the standard morphological and biochemical characteristics, HB3 isolate that showed maximum decolorization of Trypan blue was identified as Bacillus sp. HB3 isolate showed 96.6 % decolorization of Trypan blue within 24 h of incubation. Maximum decolorization of Trypan blue was found to be achieved at 35 °C, neutral pH in the presence of glucose (Carbon source) and Yeast extract (Nitrogen source). The activity of azo reductase, lignin peroxidase, tyrosinase, manganese Peroxidase was investigated for their role in biodegradation of Trypan blue. Specific activity of the azoreductase enzyme was found to be 0.46 U mg -1 protein. The crude protein extract subjected to SDS-PAGE resulted in the formation of a clear band (original band) against blue back ground which indicated the location of active azoreductase enzyme
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