International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 204-211

Isolation and Identification of Protease producing Bacteria through Biodegradation of Protein content of Kitchen Wastes in Gwalior, India
Sher Singh Gill1*, Archana Shrivastav1 and Asha Mukul Jana2
1College of Life Sciences, Cancer Hospital and Research Institute, campus, Gwalior, MP, India
2Retired Scientist, DRDE, Gwalior, MP, India
*Corresponding author

The current paper investigates skimmed milk (protein) degrading bacteria found in kitchen waste for maximum skimmed milk activity and growth of bacteria at pH and optimum temperature. Sample was collected from household kitchen waste and bacterial strains were isolated using nutrient agar media. Enrichment technique was used for isolating PDB (protein degrading bacteria) strains. The objective of research was to utilize effective bacteria for degrading complex polymer protein into simpler amino acids under optimum conditions. Out of 06 isolated bacterial strains, only two were having effective protein degrading bacteria. These two isolates were screened for qualitative estimation through Skimmed milk medium and quantitatively tested by Protease activity was measured by the standard method. The optimum pH and temperature for most potent isolate was recorded as 7 pH and 37°C correspondingly. These optimum working conditions were recommended for biomass utilization.

Keywords: Kitchen waste, protein degrading bacteria, optimization, solid waste, skimmed milk protein.

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How to cite this article:

Sher Singh Gill, Asha Mukul Jana and Archana Shrivastav. 2016. Isolation and Identification of Protease producing Bacteria through Biodegradation of Protein content of Kitchen Wastes in Gwalior.Int.J.Curr.Microbiol.App.Sci. 5(10): 204-211. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.