International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 174-184

Alkaline Protease Production by Aspergillus terreus BAB-346 using Poultry Litter Waste
Gopalkumar G. Raol1*, Priyanka M. Patel1, B.V. Raol2 and Rakeshkumar R. Panchal3
1Department of Microbiology, Shri A.N. Patel P.G. Institute, Sardar Patel University,
Anand, Gujarat, India
2Department of Microbiology, Shri P. H. G. Muni, Arts and Science College,Gujarat University, Kalol, Gujarat, India
3Department of Microbiology, M.B. Patel Science College, Sardar Patel University, VallabhVidyanagar, Gujarat, India
*Corresponding author

Aspergillus terreus BAB-346 was isolated from various agro-industrial wastes dumping site of Anand region and screened for alkaline protease enzyme production. A comparative study was carried out on the production of Alkaline protease using different type of agro-industrial waste viz. Paper pulp waste, banana stem waste and poultry litter waste as substrate in submerged medium. Amongst the various isolates, A.terreus BAB-346 produced the highest enzyme activity at 120 h of incubation as 330.0 U mg-1using Poultry litter as the sole carbon source. However, produced enzyme was partially purified as 2.5 fold by Ammonium sulphate fractionation. The purified enzyme has temperature and pH optimal of 60oC and 8.0, respectively. Purified alkaline protease is tharmostable in nature and retained fully active even at end of 20 minute of incubation at 60oC. The Alkaline protease was completely inhibited by Hg+2, Ca+2, with little increase (22 %) in the activity of upon addition of Mn+2metal ions. It is envisaged that the isolate can be a potential source of Akaline protease for use as additive in industrial application like detergent industry.

Keywords: Alkaline Protease, Aspergillus terreus,Poultry litter, Agro-industrial wastes.

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How to cite this article:

Gopalkumar G. Raol, Priyanka M. Patel, B.V. Raol and Rakeshkumar R. Panchal. 2016. Alkaline Protease Production by Aspergillus terreus BAB-346 using Poultry Litter WasteInt.J.Curr.Microbiol.App.Sci. 5(10): 174-184. doi:
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