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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4190-4197
DOI: https://doi.org/10.20546/ijcmas.2018.708.438


Antibiogram of Pathogenic Urinary E.coli Isolates from Tertiary Care Hospital in Kerala
Riyaz Sheriff*, K.K. Prasobh, N. Simi and A. Neena
Department of Microbiology, Azeezia Institute of Medical Sciences & Research, Kollam, Kerala, India
*Corresponding author
Abstract:

Urinary tract infections are one of the most common causes of patient attendance in hospitals. E.coli remains the most commonly isolated pathogen causing Urinary tract infections. Antibiotic resistance is an emerging problem in developing countries. The way ahead is to streamline the utilisation of antibiotics and to implement locally relevant antibiotic policy. A total of 1152 urine samples were received during the study period. 327 samples grew monobacterial significant bacteriuria. Semi-quantitative cultures were performed. The isolates were tested for sensitivity to the following discs Ampicillin (10μg), Gentamicin (10μg), Tobramycin (10μg), Amikacin (30μg), Ciprofloxacin(5μg) Levofloxacin (5μg), Cotrimoxazole (1.25/23.75μg) Amox-Clavulanic Acid (20/10μg) Piperacillin-Tazobactum (100/10μg) Norfloxacin (10μg) Amikacin (30μg) Cefuroxime (30μg), Ceftriaxone (30μg), Ceftazidime (30μg), Ceftazidime- Clavulanic Acid (30/10μg), Cefotaxime (30μg) Imipenam (10μg), Tetracycline (30μg), Nitrofurantoin (300μg). The sensitivity was recorded and reported as per the CLSI 2017 guidelines. Antibiotic sensitivity profile of E.coli shows sensitivity to Imipenam (91%), Nitrofurantoin (97%), Amikacin (91%), Tobramycin (72%), Piperacillin-Tazobactum (77%), Gentamicin (66%) and Tetracycline (46%). E.coli isolated exhibited resistance to ampicillin, amoxicillin-clavulanic acid, 2nd and 3rd generation cephalosporins and fluroquinolones. E.coli was sensitive to cotrimoxazole in 49% isolates. 62 (48.1%) isolates were resistant to 3 or more antibiotic groups (MDR). 17 of the 148 isolates exhibited Extended drug resistant (XDR) E.coli showed high degree of resistance to cephalosporins and fluroquinolones. One of the short term measures advised is antibiotic cycling to prolong the usefulness of live saving drugs. For this every healthcare unit needs to develop their own antibiogram to have a clear picture of the antibiotic resistance pattern prevalent in the area.


Keywords: Urinary E.coli, Antibiotic resistance, E.coli, Urinary tract infection, Antibiotic sensitivity profile
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How to cite this article:

Riyaz Sheriff, K.K. Prasobh, N. Simi and Neena, A. 2018. Antibiogram of Pathogenic Urinary E.coli Isolates from Tertiary Care Hospital in Kerala.Int.J.Curr.Microbiol.App.Sci. 7(8): 4190-4197. doi: https://doi.org/10.20546/ijcmas.2018.708.438