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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4058-4063
DOI: https://doi.org/10.20546/ijcmas.2018.708.421


Efficacy of Bacterial Collagenase Therapy in RFM Cows Assessed through Endometrial Biopsy
P. Mohan1*, K. Krishnakumar2, K. Kulasekar3, S.M.K. Karthickeyan4 and M. Murugan5
1Department of Veterinary Gynaecology and Obstetrics, Veterinary College, Shivamogga - 577 204, KVAFSU, Karnataka, India
2Cattle and Buffalo Breeding Unit, Post Graduate Research Institute in Animal Sciences, Kattupakkam-603203, Tamil Nadu Veterinary and Animal Sciences University, T.N., India
3Department of Veterinary Gynaecology and Obstetrics
4Department of Animal Breeding Genetics, Madras Veterinary College, Vepery-600 007, Tamil Nadu Veterinary and Animal Sciences University, T.N., India
5VUTRC, Melmaruvathur, Tamil Nadu Veterinary and Animal Sciences University, T.N., India
*Corresponding author
Abstract:

The study was conducted in cases of RFM presented within 12 to 24 hours after parturition to Obstetrics Unit of Madras Veterinary College, Chennai. Group I received placebo treatment with one litre of normal saline intravenously. Group II cows, treated with intrauterine proteolytic bolus containing nitrofurazone, metronidazole and urea and antibiotic therapy (Inj. Streptopenicillin @ 20,000 units/kg body weight) without manual removal for 7 days. Groups III cows, received single dose of 2, 00,000 CDU of collagenase plus 40 mg of calcium chloride and 40 mg of sodium bicarbonate dissolved in one litre of normal saline at a pH of 7.5 intravenously through jugular vein (Eiler and Hopkins,1993). Group IV received single dose of 2, 00,000 U of collagenase intravenously. The endometrial histopathology findings on day 14 and 35 in group I revealed mild to moderate glandular activity with haemorrhage and vascular activity, hyperplasic changes in the mucosa and infiltration of mononuclear cells. The higher degree of focal necrosis of epithelial mucosa and infiltration of neutrophils were recorded in group II and IV than group I and III; group II had severe fibrotic necrosis than group IV. Group II had severe diffused cellular infiltration and increased inflammatory response. Group III had mild to moderate glandular activity, infiltration of bacterial colonies, mild necrotic tissues, neutrophilic and lymphocytic infiltration; group IV had higher leucocytic infiltration, bacterial load and focal necrosis than group III. These histopathological results were revealed that the administration of collagenase through intravenous route had achieved better healing and recovery.


Keywords: Uterine tissue biopsy, RFM, Bacterial collagenase response, Cows
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How to cite this article:

Mohan, P., K. Krishnakumar, K. Kulasekar, S.M.K. Karthickeyan and Murugan, M. 2018. Efficacy of Bacterial Collagenase Therapy in RFM Cows Assessed through Endometrial Biopsy.Int.J.Curr.Microbiol.App.Sci. 7(8): 4058-4063. doi: https://doi.org/10.20546/ijcmas.2018.708.421