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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3898-3908
DOI: https://doi.org/10.20546/ijcmas.2018.708.401


Rapid Detection of Sialidase Activity for the Diagnosis of Bacterial Vaginosis
Jari Intra1, Cecilia Sarto1, Natalia Tiberti2, Claudia Siracusa1, Cinzia Savarino1, Chiara Fania3 and Paolo Brambilla1
1Department of Laboratory Medicine, University of Milano-Bicocca, Desio Hospital, via Mazzini 1, 20833, Desio (MB), Italy
2Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy
3Dipartimento di Medicina e Chirurgia, Università degli Studi Milano-Bicocca, Milano, Italy
*Corresponding author
Abstract:

Bacterial vaginosis (BV) is one of the most frequent causes of vaginal discharge in women during reproductive age worldwide. This disease is characterized by the replacement of the normal vaginal flora with an overgrowth of anaerobic bacteria most of them producing sialidase enzyme. BV is associated with an increased risk of adverse outcomes in pregnancy and susceptibility to several sexually transmitted diseases. In the present study, we evaluated the detection of sialidase activity by OSOM BVBlue test in association with routine microbial cultures and Nugent’s score, considered as the gold standard, for the diagnosis of bacterial vaginosis. Three vaginal swabs were collected from 352 women older than 12 years in age. A swab collected into Amies transport medium was employed for standard microbial cultures, a FLOQSwab for Gram stain, and a second FLOQSwab for the BVBlue test. According to Nugent’s score, BV frequency was 16.5 % (58 samples). The sensitivity of microbial culture and BVBlue test, when compared with Nugent’s score, was 69.8 % and 39.6 %, respectively. However, BVBlue test detected five cases with no bacterial growth in culture, whereas 14 samples with bacterial cultures positive for Gardnerella vaginalis showed a BVBlue test negative. The combination of microbial culture and BVBlue test increased the sensitivity to 75 % compared with Nugent’s score. In conclusion, BVBlue test alone appears not to be an efficient screening test, but, when associated with microbial cultures, can improve the diagnosis of BV.


Keywords: Bacterial vaginosis, Nugent’s score, OSOM BVBlue test, Vaginal microbial cultures, Bacterial sialidase
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How to cite this article:

Jari Intra, Cecilia Sarto, Natalia Tiberti, Claudia Siracusa, Cinzia Savarino, Chiara Fania and Paolo Brambilla. 2018. Rapid Detection of Sialidase Activity for the Diagnosis of Bacterial Vaginosis.Int.J.Curr.Microbiol.App.Sci. 7(8): 3898-3908. doi: https://doi.org/10.20546/ijcmas.2018.708.401