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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 99-108
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.012


Production of Cellulase in Submerged Fermentation using Water Hyacinth as Carbon Source and Reutilization of Spent Fungal Biomass for Dye Degradation
C.Pothiraj1*, R. Arumugam2 and R.M. Gobinath2
1Department of Botany, Government Arts College, Melur – 625 003, Tamilnadu, India
 2PG & Research Department of Botany, Alagappa Government Arts College,Karaikudi – 630 003, Tamilnadu, India
*Corresponding author
Abstract:

Cellulase enzyme production using water hyacinth biomass (WHB) as carbon source in submerged fermentation by Trichoderma viride and Aspergillus niger was studied. The FTIR spectral studies revealed that WHB had fermentable content of cellulose and hemicellulose. The fungal strains T. viride and A. niger, produced maximum cellulase at optimum culture condition with a substrate concentration of 5% (w/v) and incubation period of 6 days (72hrs). The partially purified crude enzyme obtained from the 72 hr culture of T. viride showed total cellulase activity (FPase) of 68.3 (IU) whereas, A. niger showed 46.3 (IU). The maximum CMCase enzyme production of 98.4 (IU) was exhibited by T. viride followed by A. niger 72.3 (IU). The spent fungal biomass from cellulase production media was reutilized for dye degradation potential. The present study showed that both the fungal spent biomass had moderate dye de-colourization (66%) potential and significant laccase activity (3.4 IU).


Keywords: Water hyacinth, Trichoderma viride and Aspergillus niger, Cellulase,laccase.
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How to cite this article:

Pothiraj, C., R. Arumugam and Gobinath, R.M. 2016. Production of Cellulase in Submerged Fermentation using Water Hyacinth as Carbon Source and Reutilization of Spent Fungal Biomass for Dye DegradationInt.J.Curr.Microbiol.App.Sci. 5(10): 99-108. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.012