National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cellulase enzyme production using water hyacinth biomass (WHB) as carbon source in submerged fermentation by Trichoderma viride and Aspergillus niger was studied. The FTIR spectral studies revealed that WHB had fermentable content of cellulose and hemicellulose. The fungal strains T. viride and A. niger, produced maximum cellulase at optimum culture condition with a substrate concentration of 5% (w/v) and incubation period of 6 days (72hrs). The partially purified crude enzyme obtained from the 72 hr culture of T. viride showed total cellulase activity (FPase) of 68.3 (IU) whereas, A. niger showed 46.3 (IU). The maximum CMCase enzyme production of 98.4 (IU) was exhibited by T. viride followed by A. niger 72.3 (IU). The spent fungal biomass from cellulase production media was reutilized for dye degradation potential. The present study showed that both the fungal spent biomass had moderate dye de-colourization (66%) potential and significant laccase activity (3.4 IU).
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