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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3709-3719
DOI: https://doi.org/10.20546/ijcmas.2018.708.376


Ethanol Production from Paddy Straw using Partially Purified Fungal Cellulase
Monika Agarwal1, Annu Goel2* and Leela Wati3
3Principal Scientist
1Department of Microbiology, CCS Haryana Agricultural University, Hisar-125 004, India
2Research Associate, Central Pollution Control Board, Delhi 110032, India
*Corresponding author
Abstract:

In an endeavour of exploring alternative energy sources to petroleum based fuels, bio-ethanol (ethanol derived from biomass) is considered as the most promising renewable fuel because of its potential to cut greenhouse gas emissions by 86% and higher octane (ability to resist compression) rating than gasoline. Present investigation was aimed at bio-conversion of paddy straw to ethanol using partially purified fungal cellulases. A variety of soil samples were tested for the presence of cellulolytic fungal strains using enrichment culture technique. Fungal strains were selected based on the diameter of clearance zone on carboxymethylcellulose (CMC) agar plates. Selected strains were tested for the cellulase viz., exoglucanase and endoglucanase activities before and after partial purification. Out of the 10 selected cellulolytic fungal isolates, F-1 isolate has the highest 0.42 IU/ml exoglucanase and 1.66 IU/ml endoglucanase activities. Enzyme production was maximum in Mandels and Sternberg medium containing delignified paddy straw as carbon source at 30°C after 7 days’ incubation with0.66 and 2.52 IU/ml exoglucanase and endogluanase activities, respectively. Ammonium sulfate saturation at 50-55% followed by dialysis resulted in the partial purification of crude cellulase enzyme with2.8 and 2.1 folds’ increase in exoglucanase and endogluanase activities, respectively. Hydrolysis of delignified paddy straw using partially purified enzyme obtained from F-1 isolate resulted in 63.7% solubilization of polysaccharide fraction at 500C after 4 h reaction time.


Keywords: Agricultural wastes, Bio-ethanol, Cellulase, Lignocellulosic, Paddy straw.
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How to cite this article:

Monika Agarwal, Annu Goel and Leela Wati. 2018. Ethanol Production from Paddy Straw using Partially Purified Fungal Cellulase.Int.J.Curr.Microbiol.App.Sci. 7(8): 3709-3719. doi: https://doi.org/10.20546/ijcmas.2018.708.376