International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:10, October, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 79-90

Potential Use of Azotobacter and Phosphate Solubilizing Bacteria as Biofertilizer
A.Soma Prabha*
School of Biotechnology, Madurai Kamaraj University, Madurai-21, TamilNadu, India
*Corresponding author

In the present investigation rhizosphere soil was collected in and around Chennai. The rhizosphere soils were serially diluted and pour plated on selected medium of pikovskay’s media and winogradsky’s media for isolation of bacteria. The isolated bacteria were identified based on morphological and biochemical tests. The results were compared with Bergy’s manual of determinative bacteriology. Screening is done by growth promoting bacteria by seed germination. The efficiency on different crop plants in terms of growth and yield both length and biomass. From the results, it was noted as Azotobacter and Streptomyces. Molecular characterization of microbial isolates studied by 16S RNA sequencing. Azotobacter and Rastolnia sp., were mass cultured and used in the experiment of plants and in probiotic fishes. The length and weight was measured in plant and fish. The isolated bacteria were inoculated in nutrient broth and fungus was inoculated in potato dextrose broth and it is extracted by using ethanol. Artimia lethality test was done by using brain shrimp in 96 titre well plate by selected organisms in bacteria and in fungi. The antibacterial activity test was done by kirbby boyer method in Azotobacter and phosphate solubilizing bacteria.

Keywords: Rhizosphere, Azotobacter, Rastolnia sp, PGPR, and Artimia lethality.

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How to cite this article:

Soma Prabha, A. 2016. Potential Use of Azotobacter and Phosphate Solubilizing Bacteria as Biofertilizer.Int.J.Curr.Microbiol.App.Sci. 5(10): 79-90. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.