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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2018.7(8): 3574-3582
DOI: https://doi.org/10.20546/ijcmas.2018.708.361


Molecular Characterization and Phenotypic Study of New Pleurotus djamor Isolate KKM 1
T. Praveen, R. Reihana, V.K. Parthiban and V. Ramamoorthy*
Department of Plant Pathology, Agricultural College and Research Institute, (Tamil Nadu Agricultural University) Killikulam, Vallanadu, Thoothukudi (Dist), Tamil Nadu, India
*Corresponding author
Abstract:

Recently we found new Pleurotus sp. isolate KKM 1 that grew on wood logs. The experiments were conducted to study its morphological and phenotypic characters of mycelium and basidiocarps of Pleurotus isolate KKM 1 compared with other cultivated mushroom varieties such as P. eous var. APK1, and P. djamor var. MDU1 and P. florida. Pleurotus isolate KKM 1 was confirmed as Pleurotus djamor by ITS sequencing of rDNA region. Thus, the new Pleurotus sp. isolate KKM1 was named as P. djamor isolate KKM1.The mycelial growth of P. djamor isolate KKM 1appeared loose, non-rhizomorphic type and thin mycelium whereas other cultivated mushroom varieties such as P. djamor var. MDU1 and P. florida produced compact and rhizomorphic type of mycelium. Basidiocarps of P. djamor isolate KKM 1 was distinct from other Pleurotus spp. P. djamor isolate KKM 1produced fruiting bodies with rudimentary stipe or no stipe at all, whereas other tested Pleurotus spp. have visible stipe. Pileus diameter was the maximum in P. djamor isolate KKM1.P. djamor isolate KKM1 produced leathery type of pileus having less plectenchymatous tissue and its thickness is the minimum among the tested Pleurotus spp. P. eous var. APK1 produced pink coloured fruiting bodies whereas other species produced white colored basidiocarp. P. djamor isolate KKM 1 produced pileus with wavy margins/edges whereas in other Pleurotus spp. the margin/edges are smooth. Thus the present study shows the new P. djamor isolate KKM would be a potential isolate for mushroom cultivation and mushroom germplasm collection.


Keywords: Pleurotus, Molecular characterization, ITS, Phenotype analysis
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How to cite this article:

Praveen, T., R. Reihana, V.K. Parthiban and Ramamoorthy, V. 2018. Molecular Characterization and Phenotypic Study of New Pleurotus djamor Isolate KKM 1.Int.J.Curr.Microbiol.App.Sci. 7(8): 3574-3582. doi: https://doi.org/10.20546/ijcmas.2018.708.361