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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 4368-4373
DOI: https://doi.org/10.20546/ijcmas.2018.707.509


Biogenic Synthesis of Silver Nanoparticles Using Phyllosphere Associated Bacterial Strain - Pseudomonas fluorescens
A. Tamilarasi1, G. Chitra1, K. Hemalatha1, B. Usha1, V. Pushpalatha1, M. Aufrin Taj1, Kayeen Vadakkan2, G. Ramya2, A. Vidhya1, S. Arunadevi1 and J. Hemapriya1*
1D. K. M College for Women (Autonomous), Department of Microbiology, Vellore, Tamil Nadu, India 2Bioresource Technology Lab, Department of Biotechnology, Thiruvalluvar University, Vellore, Tamil Nadu, India
*Corresponding author
Abstract:

Biogenic synthesis of silver nanoparticles using microorganisms such as actinomycetes, fungi and bacteria has attained great interest and importance because of their potential to synthesize nanoparticles of various morphologies. This study focused on the biosynthesis of silver nanoparticles using the culture supernatant of bacterial strain BRTSI-2 isolated from the Phyllosphere. Molecular identification of the isolate BRTSI-2 by16S rDNA sequencing identified the strain as Pseudomonas fluorescens. The culture condition was optimized for maximizing the bacterial biomass and was found to be effective at 30 oC at neutral pH. On treating the bacterial supernatant with 1mM final concentration of AgNO3 silver nanoparticles were formed, which was visually confirmed by the color change and using UV absorbance between 400-450 nm. FTIR analysis also supported the formation of silver nanoparticles from the bacterial supernatant.


Keywords: FTIR, Phyllosphere, Pseudomonas fluorescens, Silver nanoparticle
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How to cite this article:

Tamilarasi A., G. Chitra, K. Hemalatha, B. Usha, V. Pushpalatha, M. Aufrin Taj, Kayeen Vadakkan, G. Ramya, A. Vidhya, S. Arunadevi and Hemapriya J. 2018. Biogenic Synthesis of Silver Nanoparticles Using Phyllosphere Associated Bacterial Strain - Pseudomonas fluorescens.Int.J.Curr.Microbiol.App.Sci. 7(7): 4368-4373. doi: https://doi.org/10.20546/ijcmas.2018.707.509