National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Biogenic synthesis of silver nanoparticles using microorganisms such as actinomycetes, fungi and bacteria has attained great interest and importance because of their potential to synthesize nanoparticles of various morphologies. This study focused on the biosynthesis of silver nanoparticles using the culture supernatant of bacterial strain BRTSI-2 isolated from the Phyllosphere. Molecular identification of the isolate BRTSI-2 by16S rDNA sequencing identified the strain as Pseudomonas fluorescens. The culture condition was optimized for maximizing the bacterial biomass and was found to be effective at 30 oC at neutral pH. On treating the bacterial supernatant with 1mM final concentration of AgNO3 silver nanoparticles were formed, which was visually confirmed by the color change and using UV absorbance between 400-450 nm. FTIR analysis also supported the formation of silver nanoparticles from the bacterial supernatant.
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