National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Textile effluent samples collected from dye contaminated area of Ranipet, Vellore have been used for isolating bacterial strains. Among various bacterial isolates, BRTSI-3 was selected and was further characterized using morphological and biochemical analysis. 16S rDNA sequencing confirmed the strain BRTSI3 as Bacillus subtilis (NCBI accession number MH412808). The culture conditions for maximizing bacterial biomass were found to be optimized at 35oC and pH 8.0. Bacillus subtilis effectively decolorized methyl orange in nutrient broth within 48 h of incubation. Spectrometric methods such as UV- Vis spectrophotometry and FTIR were used for assessing the decolorization extent of methyl orange by BRTSI3. FTIR results confirmed the breakdown of methyl orange by bacterial metabolites. The investigation proved that the microorganisms found in textile effluent are capable of decolorizing and degrading the azo compounds of textile effluent.
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