International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 4361-4367

Bioremediation of Recalcitrant Textile Azo Dye - Methyl Orange by Bacillus subtilis BRTSI-3 Isolated from Textile Effluents
M.Meenatchi1, K. Shilpa1, D. Nithya1, K. Soniya1, S. Sunila1, S. Nandhini1, Kayeen Vadakkan2, A. Vidhya1, S. Ramya1 and J. Hemapriya1*
1Department of Microbiology, D. K. M College for Women, Vellore, Tamil Nadu, India 2Bioresource Technology Lab, Department of Biotechnology, Thiruvalluvar University, Vellore, Tamil Nadu, India
*Corresponding author

Textile effluent samples collected from dye contaminated area of Ranipet, Vellore have been used for isolating bacterial strains. Among various bacterial isolates, BRTSI-3 was selected and was further characterized using morphological and biochemical analysis. 16S rDNA sequencing confirmed the strain BRTSI3 as Bacillus subtilis (NCBI accession number MH412808). The culture conditions for maximizing bacterial biomass were found to be optimized at 35oC and pH 8.0. Bacillus subtilis effectively decolorized methyl orange in nutrient broth within 48 h of incubation. Spectrometric methods such as UV- Vis spectrophotometry and FTIR were used for assessing the decolorization extent of methyl orange by BRTSI3. FTIR results confirmed the breakdown of methyl orange by bacterial metabolites. The investigation proved that the microorganisms found in textile effluent are capable of decolorizing and degrading the azo compounds of textile effluent.

Keywords: Azo Dyes, Bacillus subtilis, Decolorization, Effluent, Methyl orange

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How to cite this article:

Meenatchi M., K. Shilpa, D. Nithya, K. Soniya, S. Sunila, S. Nandhini, Kayeen Vadakkan, A. Vidhya, S. Ramya and Hemapriya J. 2018. Bioremediation of Recalcitrant Textile Azo Dye - Methyl Orange by Bacillus subtilis BRTSI-3 Isolated from Textile Effluents.Int.J.Curr.Microbiol.App.Sci. 7(7): 4361-4367. doi:
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