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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 50-56
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.007


Green Synthesis of Silver Nano Particles from Aspergillus niger (MTCC-961)
P. Kalyani, K. Chandana Vineela, S. Geetha* and K.P.J. Hemalatha
Department of Microbiology, Andhra University Visakhapatnam -530 003, India
*Corresponding author
Abstract:

The silver nanoparticles synthesized biologically have been widely used in medicinal field. The synthesis of silver nanoparticles has been carried out by using the extracellular filtrate of the fungal strain. In this study, silver nanoparticles were synthesized using the fungus Aspergillus niger with an aqueous solution of AgNO3. Synthesized silver nanoparticles (Ag-NPs) were characterized through UV-visible spectrophotometer and Fourier Transform Infrared Spectroscopy (FTIR). Maximum absorbance was observed at 420 nm in visible region corresponding to the Plasmon resonance of silver nanoparticles, thus, confirming their presence. The Fourier transform infrared spectroscopy confirmed the presence of protein as stabilizing agent surrounding the silver nanoparticles. The reduction of silver ions was due to amino groups of proteins and other functional groups in the cell free filtrate of fungi. The reduction of silver ions leads to the formation of stable protein capped silver nanoparticles. The Ag-NPs and Ag-NPs + Chloramphenicol (Ab) possess potential antimicrobial activity against to Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Streptococcus sp.


Keywords: Ag-NPs, A. niger, UV- visible spectrophotometer, FTIR, Antibacterial activity.
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How to cite this article:

Kalyani, P., K. Chandana Vineela, S. Geetha and Hemalatha, K.P.J. 2016. Green Synthesis of Silver Nano Particles from Aspergillus niger (MTCC-961).Int.J.Curr.Microbiol.App.Sci. 5(10): 50-56. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.007