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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2947-2959
DOI: https://doi.org/10.20546/ijcmas.2018.708.313


Optimization of Media Components for Production of α-L-rhamnosidase from Clavispora lusitaniae KF633446
Pratiksha Singh1, Param Pal Sahota2 and Rajesh Kumar Singh1
1AgriculturalCollege, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530005, China
2Punjab Agricultural University, Ludhiana-141004, India
*Corresponding author
Abstract:

Rhamosidase producing yeast strain 84 was isolated from whey beverage and identified as Clavispara lusitaniae KF633446. The effect of different carbon sources (rhamnose, glycerol, lactose, fructose, glucose and sucrose), nitrogen sources (yeast extract, peptone, ammonium chloride, ammonium sulphate, urea and casein), temperature (10-60°C) and pH (3-8) were studied to optimize the production of rhamnosidase enzyme from Clavispara lusitaniae 84. Further, a multivariate response surface methodology evaluated the effects of different factors on enzyme activity and optimizing enzyme production. The fit of the model (R2= 0.409479) was found to be significant. Results indicated that yeast showing maximum rhamnosidase activity (0.106 IU mL-1) in presence of rhamnose (0.6% w/v), yeast extract (0.4% w/v), temperature (35±5 °C) and pH (4) in the minimal medium supplemented with naringin (0.2% w/v).


Keywords: Rhamnosidase activity, Clavispara lusitaniae, Optimize, Response surface methodology
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How to cite this article:

Pratiksha Singh, Param Pal Sahota and Rajesh Kumar Singh. 2018. Optimization of Media Components for Production of α-L-rhamnosidase from Clavispora lusitaniae KF633446.Int.J.Curr.Microbiol.App.Sci. 7(8): 2947-2959. doi: https://doi.org/10.20546/ijcmas.2018.708.313