National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Huanglongbing (HLB), also known as the “greening disease” is a serious disease affecting major citrus growing groves in India as well as in the world. The presumptive causal agent is a fastidious, gram negative, α- subdivision of the proteobacteria named Candidatus Liberibacter asiaticus (CLas) transmitted by Asian citrus psylla (Diaphorina citri). Different symptoms varying from yellowing of leaves, whole branch (called yellow shoot disease in China),drying of twigs, yellow patches in newly emerged flushes, irregular patches of yellowing on leaves (blotchy mottle), rabbit ear like appearance of leave and mineral deficiency like symptoms (leave showing symmetrical pattern of chlorosis on both sides of mid rib) were mainly observed on different citrus species during the survey conducted at various citrus growing pockets of Manipur in the present study. The leaves of citrus tree exhibiting the symptoms akin to mineral deficiency and rabbit ear like appearance were tested positive for HLB infection. Out of total 40 samples tested from different parts of Manipur by normal conventional PCR using specific primer targeting partial 16S rDNA, 19 samples were tested positive (47.50% infection) indicating high prevalence of HLB in different parts of Manipur. Highest percent infection of HLB was observed in Kachai Village, Ukhrul district, Manipur (72.72%) followed by Noney region of Tamenglong District (50%of tested samples were positive) and lowest incidence was recorded from Imphal West and East districts of Manipur (33.33%of tested samples were positive). These findings indicated widespread prevalence of HLB in different citrus growing pockets of Manipur.
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