International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:8, August, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 2427-2433

Survey and Detection of Candidatus Liberibacter asiaticus Causing Huanglongbing (HLB) Disease of Citrus in Manipur
Y. Herojit Singh1, Susheel Kumar Sharma2, Bireswar Sinha1, Ph. Sobita Devi1, K.Mamocha Singh1, A. Sapana Devi2 and K. Sarda Devi1
1Department of plant Pathology, College of Agriculture, Central Agricultural University, Imphal, India
2ICAR Research Complex for NEH Region, Manipur Centre, Imphal-795004, India
*Corresponding author

Huanglongbing (HLB), also known as the “greening disease” is a serious disease affecting major citrus growing groves in India as well as in the world. The presumptive causal agent is a fastidious, gram negative, α- subdivision of the proteobacteria named Candidatus Liberibacter asiaticus (CLas) transmitted by Asian citrus psylla (Diaphorina citri). Different symptoms varying from yellowing of leaves, whole branch (called yellow shoot disease in China),drying of twigs, yellow patches in newly emerged flushes, irregular patches of yellowing on leaves (blotchy mottle), rabbit ear like appearance of leave and mineral deficiency like symptoms (leave showing symmetrical pattern of chlorosis on both sides of mid rib) were mainly observed on different citrus species during the survey conducted at various citrus growing pockets of Manipur in the present study. The leaves of citrus tree exhibiting the symptoms akin to mineral deficiency and rabbit ear like appearance were tested positive for HLB infection. Out of total 40 samples tested from different parts of Manipur by normal conventional PCR using specific primer targeting partial 16S rDNA, 19 samples were tested positive (47.50% infection) indicating high prevalence of HLB in different parts of Manipur. Highest percent infection of HLB was observed in Kachai Village, Ukhrul district, Manipur (72.72%) followed by Noney region of Tamenglong District (50%of tested samples were positive) and lowest incidence was recorded from Imphal West and East districts of Manipur (33.33%of tested samples were positive). These findings indicated widespread prevalence of HLB in different citrus growing pockets of Manipur.

Keywords: Blotchy mottle, Citrus Psylla, Huanlongbing, Incidence, Prevalence

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How to cite this article:

Herojit Singh Y., Susheel Kumar Sharma, Bireswar Sinha, Ph. Sobita Devi, K. Mamocha Singh, A. Sapana Devi and Sarda Devi, K. 2018. Survey and Detection of Candidatus Liberibacter asiaticus Causing Huanglongbing (HLB) Disease of Citrus in Manipur.Int.J.Curr.Microbiol.App.Sci. 7(8): 2427-2433. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.