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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 1912-1921
DOI: https://doi.org/10.20546/ijcmas.2018.708.220


Phenotypic Characteristics of Yeasts of the Genus Candida and Cryptococcus in Differential Culture Media
Larissa Alves Lima, Mylla Augusta Silva Faria, Ralciane de Paula Menezes, Mário Paulo A. Penatti and Reginaldo dos Santos Pedroso*
Technical Course in Clinical Analysis, Technical School of Health, Federal University of Uberlandia, Campus Umuarama, Uberlandia, Minas Gerais, Brazil
*Corresponding author
Abstract:

In the clinical mycology laboratory, the identification of yeast species is done by screening in specific media, such as chromogenic agar for Candida species and Niger seed agar for Cryptococcus species, both of which are of clinical interest. This study aimed to evaluate the growth and morphological characteristics of yeasts of the Candida and Cryptococcus species in different culture media. Yeast species included in the study were: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. lipolytica, C. parapsilosis, C. metapsilosis, C. orthopsilosis, C. neoformans, C. gattii, C. flavescens, and C. albidus. The media used were Sabouraud dextrose agar, Sabouraud dextrose broth, hypertonic Sabouraud broth (plus 6.5% NaCl), Candida chromogenic agar, methyldopa agar, Niger seed agar and tobacco agar. Growth, color, size, presence of fringes, melanin and the appearance of the colony were evaluated. All isolates grew in the media used, except for the hypertonic Sabouraud broth; in Candida chromogenic agar, C. albicans and C. dubliniensis present a green color and C. tropicalis a blue color, while other species show colors including pink, purple, gray and white; in the Niger seed agar, C. neoformans, C. gattii and C. flavescens presented a brown color, while others had white colonies; in tobacco agar, the colors included white, cream and gray; and in methyldopa agar, all colonies were white. Some isolates presented colonies with fringes in the tobacco, methyldopa and Niger seed agar; the presence of melanin was observed by Cryptococcus isolates in the Niger seed and tobacco agar; the appearance of colonies in the media varied from opaque to shiny or mucoid, according to the isolate and the culture medium. All of the culture media used allowed the growth of the tested isolates, except for C. lipolytica, which did not grow in hypertonic Sabouraud broth. The isolates of Cryptococcus, C. krusei and C. dubliniensis presented a significant reduction of growth in hypertonic Sabouraud broth.


Keywords: Culture media, Yeasts, Cryptococcus, Candida
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How to cite this article:

Larissa Alves Lima, Mylla Augusta Silva Faria, Ralciane de Paula Menezes, Mário Paulo A. Penatti and Reginaldo dos Santos Pedroso. 2018. Phenotypic Characteristics of Yeasts of the Genus Candida and Cryptococcus in Differential Culture Media.Int.J.Curr.Microbiol.App.Sci. 7(8): 1912-1921. doi: https://doi.org/10.20546/ijcmas.2018.708.220