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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 816-825
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.092


Analysis of Bacterial Community in the Rhizosphere of Rice subjected to Heavy Metal Pollution
Ding Zili1,2, Wu Jinping2, Jiao Chunhai2 and Cao Cougui1*
1College of Plant Science and Technology, Huazhong Agricultural University, Wuhan43070, China
2Hubei Academy of Agricultural Sciences, Wuhan430064, China
*Corresponding author
Abstract:

PCR amplification of 16S rRNA genes and denaturing gradient gel electrophoresis (DGGE) analysis were used to examine the compositions and dynamics of bacterial communities in the rhizosphere of rice subjected to heavy metal pollution. DGGE showed many common bands under conditions of Cd and Cr pollution, suggesting the presence of similar of bacterial communities. Principal component analysis showed different microbial community structure changes during the study period, for Cd and Cr pollution respectively. Major bands of DGGE patterns were sequenced. Phylogenetic analysis indicated that the majority of the sequences obtained were affiliated with members of Acidobacteria (18.75 %), Myxococcales (12.50 %), Gemmatimonas (12.50 %), and Thiothrix (9.38 %). When the rice plants reached the heading stage, the relative abundance of members of Gemmatimon as increased with Cd concentration, while abundance of members of Gallionellaceae increased with Cr concentration. At the maturation stage, the relative abundance of members of Chlorobiales increased with Cd concentration. These results illuminate the effect on diversity of heavy metal pollution in soil. Further study of combinations of other environmental parameters will clarify heavy metal effects on the activity of microbial communities in rice paddy fields.


Keywords: Soil pollution; Heavy metals; Rhizosphere; PCR-DGGE; Microbial community
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How to cite this article:

Ding Zili, Wu Jinping, Jiao Chunhai, Cao Cougui. 2016. Analysis of Bacterial Community in the Rhizosphere of Rice subjected to Heavy Metal Pollution.Int.J.Curr.Microbiol.App.Sci. 5(9): 816-825. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.092