Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 506-517
DOI: https://doi.org/10.20546/ijcmas.2018.708.056


The Effect of Exogenous HSP70 on Expression Kinetics of HSP70, HSP90, HSP110 and IL2 and IL6 in Peripheral Blood Mononuclear Cells and Possible Crosstalk between Them in Black Bengal Goat
Tanmay Mondal1, Chandrabhan Singh Banjare1, Satyaveer Singh Dangi1, Sadhan Bag1, Mihir Sarkar1 and Bikash Chandra Das2*
1Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India
2Eastern Regional Station, Indian Veterinary Research Institute, 37 Belgachia Road,
Kolkata-700037, India
*Corresponding author
Abstract:

The present study was aimed to investigate the modulatory effect of eHSP70 on major heat stressed genes in in-vitro cell culture system. HSP70 (eHSP70) on mRNA expression profile of Heat shock protein 70 (HSP70), Heat shock protein 90 (HSP90), Heat shock protein 110 (HSP110), Interleukin-2 (IL-2), Interleukin-6 (IL-6) in peripheral blood mononuclear cells (PBMC) in cell culture model of black Bengal Goat. The cultured PBMCs were treated with a specific dose of eHSP70 (50 ng/ml) at 37°C for 0, 1, 2, 4, 8, 12, 16, 20, 24 hours. The mRNA expression of the above mentioned genes were determined by quantitative real-time PCR (qRT-PCR). The proteins were immunochemically localized by the cultured PBMCs. The study showed that on administration of eHSP70 to the PBMCs cell culture system, the relative mRNA expression of iHSP70, HSP90 and HSP110 increased significantly (p<0.05) at 4, 20 and 24 h compared with control and Interleukins (IL-2), (IL-6), showed significantly higher expression (p<0.05) at 4 and 24 h compared with control. Immunoreactivity revealed that all genes under study were localized in cytoplasm as well as in nucleus. The differential expression pattern of above mentioned genes at specific dose of eHSP70 might indicate their role in maintaining the cellular homeostasis during heat stress.


Keywords: Goat, Kinetics, Heat stress, HSP, IL, PBMC
Download this article as Download

How to cite this article:

Tanmay Mondal, Chandrabhan Singh Banjare, Satyaveer Singh Dangi, Sadhan Bag, Mihir Sarkar and Bikash Chandra Das. 2018. The Effect of Exogenous HSP70 on Expression Kinetics of HSP70, HSP90, HSP110 and IL2 and IL6 in Peripheral Blood Mononuclear Cells and Possible Crosstalk between Them in Black Bengal Goat.Int.J.Curr.Microbiol.App.Sci. 7(8): 506-517. doi: https://doi.org/10.20546/ijcmas.2018.708.056