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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 514-523
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.057


Haemagglutination and Resistance to the Bactericidal Activity of Serum as the Urovirulence Markers of Uropathogenic Escherichia coli
S.M. Nachammai1*, Karthika Jayakumar2, Vinithra Suresh3, M.Kousalya4 and Anbu N. Aravazhi5
Department of Microbiology, Karpagam Faculty of Medical Sciences and Research, Coimbatore, India
*Corresponding author
Abstract:

Bacterial strains causing extra intestinal infections harbor virulence factors that enhance the ability to cause systemic infection. Uropathogenic Escherichia coli (UPEC) responsible for UTI express a multitude of virulence factors to break the inertia of the mucosal barrier. To study the haemolysin, haemagglutination and resistance to the bactericidal activity of the serum property as urovirulence factors of E.coli and antimicrobial susceptibility pattern. 100 E.coli strains isolated from urine samples out of 400 symptomatic cases were screened for virulence factors like Haemolysin, Mannose resistant, Mannose sensitive Haemagglutination (MRHA, MSHA) and Serum resistance by phenotypic methods. Antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method as per CLSI guidelines. Among 100 E.coli strains 33% were hemolytic, 9% showed MRHA, 14% showed MSHA, there was no haemagglutination in 73% strains and 76%were serum resistant. Least percentage of resistance was seen to Imipenem (1%), Piperacillin/tazobactum (1%) and Nitrofurantoin (19%). High percentage of resistance was found to Penicillins and Norfloxacin (79%). The present study revealed that UPEC exhibited one or more virulence factors. Identifying virulence markers will definitely prevent the complications like recurrent, chronic and persistent urinary tract infections.


Keywords: Uropathogenic Escherichia coli, antimicrobial susceptibility, virulence factors, haemolysin, haemagglutination, serum resistance.
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How to cite this article:

Nachammai, S.M., Karthika Jayakumar, Vinithra Suresh, M. Kousalya and Anbu N. Aravazhi. 2016.  Haemagglutination and Resistance to the Bactericidal Activity of Serum as the Urovirulence Markers of Uropathogenic Escherichia coli.Int.J.Curr.Microbiol.App.Sci. 5(9): 514-523. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.057