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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 4308-4318
DOI: https://doi.org/10.20546/ijcmas.2018.707.502


Comparative Assessment and Optimisation of Different DNA Extraction Methods in Lesser Yam (Dioscorea esculenta)
Visalakshichandra1, M. N. Sheela1, A. S. Swathy2,B.  S. Prakash Krishnan1 and Vivek Hegde1
1ICAR-Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekaryam, Thiruvanathapuram, Kerala, India
2A.J. College of Science and Technology, Thonnakkal, Thiruvanathapuram, Kerala, India
*Corresponding author
Abstract:

The effectiveness of genotyping for any genetic studies relies on the quantity and quality of DNA isolated. The DNA isolation procedures differs for different crop species depending upon the phytochemical composition of the tissue used for isolation of DNA. The polyphenol abundance in Lesser yam interferes in the isolation of high quality DNA. The quantitative and qualitative assessment of DNA isolated using different extraction methods therefore becomes a priority. The selection of accurate isolation method becomes absolutely essential to obtain PCR amplification. In this study, five DNA extraction methods were compared in terms of quantity, quality/purity, time consumed, integrity and functionality. Among the DNA extraction methods analysed in this study, the Asemota method was found to be the most efficient in isolating high DNA yield with better quality from Dioscorea esculenta. The DNA extracted using this protocol can be used for whole-genome sequencing, advanced sequencing technologies, and bioinformatic tools.


Keywords: DNA extraction method, Dioscorea esculenta, Quality of DNA, commercial kit, PCR
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How to cite this article:

Visalakshichandra, M. N. Sheela, A. S. Swathy, B. S. Prakash Krishnan and Vivek Hegde. 2018. Comparative Assessment and Optimisation of Different DNA Extraction Methods in Lesser Yam (Dioscorea esculenta).Int.J.Curr.Microbiol.App.Sci. 7(7): 4308-4318. doi: https://doi.org/10.20546/ijcmas.2018.707.502